Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 8899..9491 Replicon plasmid p94EC-3
Accession NZ_CP047579
Organism Escherichia coli strain 94EC

Toxin (Protein)


Gene name hicA Uniprot ID A0A1I9ZJC5
Locus tag GUJ28_RS24855 Protein ID WP_000513532.1
Coordinates 8899..9147 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1I9ZJ49
Locus tag GUJ28_RS24860 Protein ID WP_000681609.1
Coordinates 9144..9491 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUJ28_RS24820 3911..4576 - 666 WP_000532149.1 P-loop NTPase -
GUJ28_RS24825 5165..5494 + 330 WP_001542022.1 hypothetical protein -
GUJ28_RS24830 5895..6410 + 516 WP_001025393.1 J domain-containing protein -
GUJ28_RS24835 6514..7056 + 543 WP_001083904.1 hypothetical protein -
GUJ28_RS24840 7053..7307 + 255 WP_001230709.1 hypothetical protein -
GUJ28_RS24845 7304..7546 + 243 WP_000151456.1 hypothetical protein -
GUJ28_RS24850 8117..8410 + 294 WP_000673428.1 hypothetical protein -
GUJ28_RS24855 8899..9147 + 249 WP_000513532.1 type II toxin-antitoxin system HicA family toxin Toxin
GUJ28_RS24860 9144..9491 + 348 WP_000681609.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GUJ28_RS24865 9579..9761 - 183 WP_001428470.1 hypothetical protein -
GUJ28_RS24870 10263..10562 + 300 WP_034169410.1 type II toxin-antitoxin system HicB family antitoxin -
GUJ28_RS24875 10822..11148 - 327 WP_000206697.1 hypothetical protein -
GUJ28_RS24880 11150..11590 - 441 WP_023223352.1 hypothetical protein -
GUJ28_RS24885 11610..12317 - 708 WP_000108794.1 hypothetical protein -
GUJ28_RS24890 12319..13071 - 753 WP_000797966.1 conjugal transfer protein TraL -
GUJ28_RS24895 13089..13490 - 402 WP_021575837.1 hypothetical protein -
GUJ28_RS24900 13858..14199 + 342 WP_000730722.1 plasmid mobilization relaxosome protein MobC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-1.1 - 1..60960 60960


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9378.03 Da        Isoelectric Point: 10.4800

>T146801 WP_000513532.1 NZ_CP047579:8899-9147 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Download         Length: 249 bp

>T146801 NZ_CP062782:c5217469-5217296 [Escherichia coli O157:H7]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13206.97 Da        Isoelectric Point: 4.8313

>AT146801 WP_000681609.1 NZ_CP047579:9144-9491 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Download         Length: 348 bp

>AT146801 NZ_CP062782:c5217793-5217497 [Escherichia coli O157:H7]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9ZJC5

Antitoxin

Source ID Structure
AlphaFold DB A0A1I9ZJ49

References