Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 1987..2534 Replicon plasmid p94EC-2
Accession NZ_CP047578
Organism Escherichia coli strain 94EC

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag GUJ28_RS24040 Protein ID WP_000969996.1
Coordinates 2253..2534 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A723YP93
Locus tag GUJ28_RS24035 Protein ID WP_021559054.1
Coordinates 1987..2256 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUJ28_RS24030 46..1077 + 1032 WP_000907868.1 replication initiation protein -
GUJ28_RS25610 1774..1932 + 159 WP_000841008.1 hypothetical protein -
GUJ28_RS24035 1987..2256 + 270 WP_021559054.1 hypothetical protein Antitoxin
GUJ28_RS24040 2253..2534 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GUJ28_RS25615 2569..2631 + 63 Protein_4 3'-5' exonuclease -
GUJ28_RS24045 2720..3004 + 285 WP_001407321.1 ribbon-helix-helix domain-containing protein -
GUJ28_RS24050 3004..3281 + 278 Protein_6 type II toxin-antitoxin system RelE/ParE family toxin -
GUJ28_RS24055 3377..3634 + 258 WP_001685860.1 hypothetical protein -
GUJ28_RS24060 3636..4343 + 708 WP_058100829.1 hypothetical protein -
GUJ28_RS24065 4347..4808 + 462 WP_024172415.1 hypothetical protein -
GUJ28_RS24070 5114..5587 - 474 WP_058100828.1 hypothetical protein -
GUJ28_RS24075 5764..6624 - 861 WP_000027057.1 class A broad-spectrum beta-lactamase TEM-1 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / aac(3)-IVa / aph(4)-Ia / tet(X4) / floR / fosA3 / blaCTX-M-65 - 1..134426 134426


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T146794 WP_000969996.1 NZ_CP047578:2253-2534 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T146794 NZ_CP062782:3879300-3879686 [Escherichia coli O157:H7]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAAGCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10222.60 Da        Isoelectric Point: 8.8735

>AT146794 WP_021559054.1 NZ_CP047578:1987-2256 [Escherichia coli]
MAQINMSLRIDAELKDAFMAAAKSMDRNASQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT146794 NZ_CP062782:3879676-3880005 [Escherichia coli O157:H7]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A723YP93

References