Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 155658..156301 Replicon plasmid p94EC-1
Accession NZ_CP047577
Organism Escherichia coli strain 94EC

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag GUJ28_RS23930 Protein ID WP_001034044.1
Coordinates 155885..156301 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag GUJ28_RS23925 Protein ID WP_001261286.1
Coordinates 155658..155888 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUJ28_RS23905 151220..151909 - 690 WP_001513524.1 RES family NAD+ phosphorylase -
GUJ28_RS23910 152328..152558 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
GUJ28_RS23915 152555..152971 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin -
GUJ28_RS23920 153133..155271 - 2139 WP_001513523.1 AAA family ATPase -
GUJ28_RS23925 155658..155888 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GUJ28_RS23930 155885..156301 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
GUJ28_RS23935 156376..157941 + 1566 WP_001128474.1 AAA family ATPase -
GUJ28_RS23940 157926..158948 + 1023 WP_000361402.1 helicase UvrD -
GUJ28_RS23945 159202..159898 - 697 Protein_167 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / sitABCD iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA 1..178137 178137
- flank IS/Tn - - 159202..159714 512


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T146793 WP_001034044.1 NZ_CP047577:155885-156301 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T146793 NZ_CP062782:3713337-3713624 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT146793 WP_001261286.1 NZ_CP047577:155658-155888 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT146793 NZ_CP062782:3713146-3713337 [Escherichia coli O157:H7]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References