Detailed information of TA system TA146792

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 152328..152971
• Replicon: plasmid p94EC-1
• Accession: NZ_CP047577
• Organism: Escherichia coli strain 94EC

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B1LRW4
• Locus tag: GUJ28_RS23915
• Protein ID: WP_001044768.1
• Coordinates: 152555..152971 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: GUJ28_RS23910
• Protein ID: WP_001261287.1
• Coordinates: 152328..152558 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUJ28_RS23880	147759..147935	-	177	Protein_154	RepB family plasmid replication initiator protein	-
GUJ28_RS23885	148635..149417	-	783	WP_001513526.1	site-specific integrase	-
GUJ28_RS23890	149418..149723	-	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	-
GUJ28_RS23895	149725..149943	-	219	WP_000813630.1	type II toxin-antitoxin system antitoxin CcdA	-
GUJ28_RS23900	150499..151188	+	690	WP_001513525.1	helix-turn-helix domain-containing protein	-
GUJ28_RS23905	151220..151909	-	690	WP_001513524.1	RES family NAD+ phosphorylase	-
GUJ28_RS23910	152328..152558	+	231	WP_001261287.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GUJ28_RS23915	152555..152971	+	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	Toxin
GUJ28_RS23920	153133..155271	-	2139	WP_001513523.1	AAA family ATPase	-
GUJ28_RS23925	155658..155888	+	231	WP_001261286.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
GUJ28_RS23930	155885..156301	+	417	WP_001034044.1	type II toxin-antitoxin system VapC family toxin	-
GUJ28_RS23935	156376..157941	+	1566	WP_001128474.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / sitABCD	iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA	1..178137	178137

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15197.66 Da
• Isoelectric Point: 7.7805

>T146792 WP_001044768.1 NZ_CP047577:152555-152971 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T146792 NZ_CP062782:c3490328-3489954 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT146792 WP_001261287.1 NZ_CP047577:152328-152558 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT146792 NZ_CP062782:c3490749-3490375 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A606Q844

Antitoxin

• AlphaFold DB: A0A7U9QFC4