Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 149418..149943 Replicon plasmid p94EC-1
Accession NZ_CP047577
Organism Escherichia coli strain 94EC

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag GUJ28_RS23890 Protein ID WP_001159871.1
Coordinates 149418..149723 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag GUJ28_RS23895 Protein ID WP_000813630.1
Coordinates 149725..149943 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUJ28_RS23865 145521..145720 + 200 Protein_151 lysis protein -
GUJ28_RS23870 145778..146071 - 294 WP_000738422.1 increased serum survival lipoprotein Iss -
GUJ28_RS23875 146528..147756 + 1229 WP_088130945.1 IS3 family transposase -
GUJ28_RS23880 147759..147935 - 177 Protein_154 RepB family plasmid replication initiator protein -
GUJ28_RS23885 148635..149417 - 783 WP_001513526.1 site-specific integrase -
GUJ28_RS23890 149418..149723 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
GUJ28_RS23895 149725..149943 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
GUJ28_RS23900 150499..151188 + 690 WP_001513525.1 helix-turn-helix domain-containing protein -
GUJ28_RS23905 151220..151909 - 690 WP_001513524.1 RES family NAD+ phosphorylase -
GUJ28_RS23910 152328..152558 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
GUJ28_RS23915 152555..152971 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / sitABCD iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA 1..178137 178137


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T146791 WP_001159871.1 NZ_CP047577:c149723-149418 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T146791 NZ_CP062782:3195958-3196065 [Escherichia coli O157:H7]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT146791 WP_000813630.1 NZ_CP047577:c149943-149725 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT146791 NZ_CP062782:c3195905-3195844 [Escherichia coli O157:H7]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References