Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4157041..4157666 Replicon chromosome
Accession NZ_CP047576
Organism Escherichia coli strain 94EC

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag GUJ28_RS19930 Protein ID WP_000911329.1
Coordinates 4157268..4157666 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag GUJ28_RS19925 Protein ID WP_000450524.1
Coordinates 4157041..4157268 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUJ28_RS19900 4152816..4153286 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
GUJ28_RS19905 4153286..4153858 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
GUJ28_RS19910 4154004..4154882 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
GUJ28_RS19915 4154899..4155960 + 1062 WP_001436054.1 outer membrane protein assembly factor BamC -
GUJ28_RS19920 4156173..4156886 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
GUJ28_RS19925 4157041..4157268 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
GUJ28_RS19930 4157268..4157666 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
GUJ28_RS19935 4157813..4158676 + 864 WP_001267498.1 neutral zinc metallopeptidase -
GUJ28_RS19940 4158691..4160706 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
GUJ28_RS19945 4160780..4161478 + 699 WP_000679823.1 esterase -
GUJ28_RS19950 4161588..4161788 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T146784 WP_000911329.1 NZ_CP047576:4157268-4157666 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T146784 NZ_CP062782:946485-946892 [Escherichia coli O157:H7]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT146784 WP_000450524.1 NZ_CP047576:4157041-4157268 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT146784 NZ_CP062782:946238-946504 [Escherichia coli O157:H7]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References