Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3682469..3683123 Replicon chromosome
Accession NZ_CP047576
Organism Escherichia coli strain 94EC

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag GUJ28_RS17615 Protein ID WP_000244772.1
Coordinates 3682716..3683123 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GUJ28_RS17610 Protein ID WP_000354046.1
Coordinates 3682469..3682735 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUJ28_RS17585 3677638..3678381 + 744 WP_000951941.1 SDR family oxidoreductase -
GUJ28_RS17590 3678438..3679871 - 1434 WP_160192077.1 6-phospho-beta-glucosidase BglA -
GUJ28_RS17595 3679916..3680227 + 312 WP_001182945.1 N(4)-acetylcytidine aminohydrolase -
GUJ28_RS17600 3680391..3681050 + 660 WP_000250274.1 hemolysin III family protein -
GUJ28_RS17605 3681246..3682226 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
GUJ28_RS17610 3682469..3682735 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GUJ28_RS17615 3682716..3683123 + 408 WP_000244772.1 protein YgfX Toxin
GUJ28_RS17620 3683163..3683684 - 522 WP_001055874.1 flavodoxin FldB -
GUJ28_RS17625 3683796..3684692 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GUJ28_RS17630 3684717..3685427 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GUJ28_RS17635 3685433..3687166 + 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T146781 WP_000244772.1 NZ_CP047576:3682716-3683123 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T146781 NZ_CP062782:c457210-456725 [Escherichia coli O157:H7]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT146781 WP_000354046.1 NZ_CP047576:3682469-3682735 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT146781 NZ_CP062782:c457479-457201 [Escherichia coli O157:H7]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References