Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3097613..3098244 Replicon chromosome
Accession NZ_CP047576
Organism Escherichia coli strain 94EC

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag GUJ28_RS14670 Protein ID WP_001259388.1
Coordinates 3097613..3097888 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag GUJ28_RS14675 Protein ID WP_000593555.1
Coordinates 3097885..3098244 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUJ28_RS14655 3092617..3093684 + 1068 WP_000361482.1 HlyD family efflux transporter periplasmic adaptor subunit -
GUJ28_RS14660 3093681..3096416 + 2736 WP_160192067.1 ribosome-associated ATPase/putative transporter RbbA -
GUJ28_RS14665 3096416..3097540 + 1125 WP_001615305.1 ABC transporter permease -
GUJ28_RS14670 3097613..3097888 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
GUJ28_RS14675 3097885..3098244 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GUJ28_RS14680 3098364..3098765 - 402 WP_001190064.1 nickel-responsive transcriptional regulator NikR -
GUJ28_RS14685 3098771..3099577 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
GUJ28_RS14690 3099574..3100338 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
GUJ28_RS14695 3100338..3101171 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
GUJ28_RS14700 3101168..3102112 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T146779 WP_001259388.1 NZ_CP047576:3097613-3097888 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T146779 NZ_CP062782:408068-408595 [Escherichia coli O157:H7]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT146779 WP_000593555.1 NZ_CP047576:3097885-3098244 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT146779 NZ_CP062782:407795-408061 [Escherichia coli O157:H7]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References