Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 2238609..2239204 Replicon chromosome
Accession NZ_CP047576
Organism Escherichia coli strain 94EC

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag GUJ28_RS10755 Protein ID WP_000239579.1
Coordinates 2238609..2238959 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag GUJ28_RS10760 Protein ID WP_001223208.1
Coordinates 2238953..2239204 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUJ28_RS10735 2234055..2235077 - 1023 WP_001368084.1 ABC transporter permease -
GUJ28_RS10740 2235091..2236593 - 1503 WP_000210554.1 sugar ABC transporter ATP-binding protein -
GUJ28_RS10745 2236733..2237689 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
GUJ28_RS10750 2237999..2238529 + 531 WP_000055075.1 inorganic diphosphatase -
GUJ28_RS10755 2238609..2238959 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
GUJ28_RS10760 2238953..2239204 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
GUJ28_RS10765 2239416..2239757 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
GUJ28_RS10770 2239760..2243539 - 3780 WP_000060896.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T146774 WP_000239579.1 NZ_CP047576:c2238959-2238609 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T146774 NZ_CP062781:2066-2341 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT146774 WP_001223208.1 NZ_CP047576:c2239204-2238953 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT146774 NZ_CP062781:1782-2066 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References