Detailed information of TA system TA146773

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2154544..2155379
• Replicon: chromosome
• Accession: NZ_CP047576
• Organism: Escherichia coli strain 94EC

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LDL9
• Locus tag: GUJ28_RS10310
• Protein ID: WP_001094444.1
• Coordinates: 2154544..2154921 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7LDN9
• Locus tag: GUJ28_RS10315
• Protein ID: WP_001285607.1
• Coordinates: 2155011..2155379 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUJ28_RS10280	2149804..2150217	+	414	WP_001368082.1	hypothetical protein	-
GUJ28_RS10285	2150214..2151683	+	1470	WP_001022619.1	serine/threonine protein kinase	-
GUJ28_RS10290	2151884..2153149	-	1266	WP_001218329.1	integrase arm-type DNA-binding domain-containing protein	-
GUJ28_RS25525	2153611..2153757	-	147	WP_001290178.1	hypothetical protein	-
GUJ28_RS10300	2153842..2154039	-	198	WP_001327226.1	DUF957 domain-containing protein	-
GUJ28_RS10305	2154059..2154547	-	489	WP_000761685.1	hypothetical protein	-
GUJ28_RS10310	2154544..2154921	-	378	WP_001094444.1	TA system toxin CbtA family protein	Toxin
GUJ28_RS10315	2155011..2155379	-	369	WP_001285607.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GUJ28_RS10320	2155459..2155680	-	222	WP_000692350.1	DUF987 domain-containing protein	-
GUJ28_RS10325	2155767..2156243	-	477	WP_001384029.1	RadC family protein	-
GUJ28_RS10330	2156258..2156743	-	486	WP_094317241.1	antirestriction protein	-
GUJ28_RS10335	2156835..2157653	-	819	WP_001175175.1	DUF945 domain-containing protein	-
GUJ28_RS10340	2157743..2157952	-	210	WP_032150870.1	DUF905 family protein	-
GUJ28_RS10345	2157982..2158659	-	678	WP_001097301.1	hypothetical protein	-
GUJ28_RS10350	2158807..2159487	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimC / fimI / fimA / fimE / fimB	2123254..2214451	91197

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14087.02 Da
• Isoelectric Point: 7.3523

>T146773 WP_001094444.1 NZ_CP047576:c2154921-2154544 [Escherichia coli]
MNTLPDTHVREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SVCTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T146773 NZ_CP062780:c5054000-5053827 [Escherichia coli O157:H7]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13689.53 Da
• Isoelectric Point: 6.4669

>AT146773 WP_001285607.1 NZ_CP047576:c2155379-2155011 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT146773 NZ_CP062780:c5054324-5054028 [Escherichia coli O157:H7]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: B1LDL9

Antitoxin

• AlphaFold DB: A0A1M0JLU8