146769

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1556628..1557465 Replicon chromosome
Accession NZ_CP047576
Organism Escherichia coli strain 94EC

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag GUJ28_RS07540 Protein ID WP_000227784.1
Coordinates 1556923..1557465 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag GUJ28_RS07535 Protein ID WP_001297137.1
Coordinates 1556628..1556939 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUJ28_RS07510 1551648..1552595 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
GUJ28_RS07515 1552617..1554608 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
GUJ28_RS07520 1554598..1555212 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
GUJ28_RS07525 1555212..1555541 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
GUJ28_RS07530 1555553..1556443 + 891 WP_000971336.1 heme o synthase -
GUJ28_RS07535 1556628..1556939 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
GUJ28_RS07540 1556923..1557465 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
GUJ28_RS07545 1557521..1558456 - 936 WP_001297127.1 sel1 repeat family protein -
GUJ28_RS07550 1558864..1560228 + 1365 WP_001000978.1 MFS transporter -
GUJ28_RS07555 1560356..1560847 - 492 WP_001138904.1 nucleotide binding protein YajQ -
GUJ28_RS07560 1561015..1561926 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T146769 WP_000227784.1 NZ_CP047576:1556923-1557465 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T146769 NZ_CP062780:4475066-4475326 [Escherichia coli O157:H7]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT146769 WP_001297137.1 NZ_CP047576:1556628-1556939 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT146769 NZ_CP062780:4474787-4475047 [Escherichia coli O157:H7]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References