Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1522552..1523170 Replicon chromosome
Accession NZ_CP047576
Organism Escherichia coli strain 94EC

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GUJ28_RS07370 Protein ID WP_001291435.1
Coordinates 1522952..1523170 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID E9TLB8
Locus tag GUJ28_RS07365 Protein ID WP_000344794.1
Coordinates 1522552..1522926 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUJ28_RS07355 1517641..1518834 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GUJ28_RS07360 1518857..1522006 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
GUJ28_RS07365 1522552..1522926 + 375 WP_000344794.1 Hha toxicity modulator TomB Antitoxin
GUJ28_RS07370 1522952..1523170 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GUJ28_RS07375 1523341..1523892 + 552 WP_000102564.1 maltose O-acetyltransferase -
GUJ28_RS07380 1524008..1524478 + 471 WP_000136192.1 YlaC family protein -
GUJ28_RS07385 1524642..1526192 + 1551 WP_001364591.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GUJ28_RS07390 1526234..1526587 - 354 WP_000878140.1 DUF1428 domain-containing protein -
GUJ28_RS07400 1526966..1527277 + 312 WP_000409911.1 MGMT family protein -
GUJ28_RS07405 1527308..1527880 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T146768 WP_001291435.1 NZ_CP047576:1522952-1523170 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T146768 NZ_CP062780:c4469067-4468669 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14527.36 Da        Isoelectric Point: 4.9284

>AT146768 WP_000344794.1 NZ_CP047576:1522552-1522926 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGRVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT146768 NZ_CP062780:c4469363-4469070 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure

References