Detailed information of TA system TA146767

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 503493..504131
• Replicon: chromosome
• Accession: NZ_CP047576
• Organism: Escherichia coli strain 94EC

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: GUJ28_RS02475
• Protein ID: WP_000813794.1
• Coordinates: 503955..504131 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GUJ28_RS02470
• Protein ID: WP_001270285.1
• Coordinates: 503493..503909 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUJ28_RS02450	498645..499586	-	942	WP_001251313.1	ABC transporter permease	-
GUJ28_RS02455	499587..500600	-	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
GUJ28_RS02460	500618..501763	-	1146	WP_000034363.1	ABC transporter substrate-binding protein	-
GUJ28_RS02465	502008..503414	-	1407	WP_000760589.1	PLP-dependent aminotransferase family protein	-
GUJ28_RS02470	503493..503909	-	417	WP_001270285.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
GUJ28_RS02475	503955..504131	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
GUJ28_RS02480	504353..504583	+	231	WP_000494244.1	YncJ family protein	-
GUJ28_RS02485	504675..506636	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
GUJ28_RS02490	506709..507245	-	537	WP_000429153.1	DNA-binding transcriptional regulator SutR	-
GUJ28_RS02495	507298..508512	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	508552..509700	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T146767 WP_000813794.1 NZ_CP047576:c504131-503955 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T146767 NZ_CP062780:4211568-4211786 [Escherichia coli O157:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15247.61 Da
• Isoelectric Point: 4.6115

>AT146767 WP_001270285.1 NZ_CP047576:c503909-503493 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT146767 NZ_CP062780:4211168-4211542 [Escherichia coli O157:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• PDB: 6HPB