Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 54557..55079 Replicon plasmid p2EC1-2
Accession NZ_CP047573
Organism Escherichia coli strain 2EC1

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag GTQ78_RS22995 Protein ID WP_000220560.1
Coordinates 54798..55079 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag GTQ78_RS22990 Protein ID WP_000121743.1
Coordinates 54557..54808 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTQ78_RS22975 49668..50309 - 642 WP_001694850.1 restriction endonuclease -
GTQ78_RS22980 50384..52693 - 2310 Protein_64 Tn3 family transposase -
GTQ78_RS22985 52747..53451 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
GTQ78_RS22990 54557..54808 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
GTQ78_RS22995 54798..55079 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GTQ78_RS23000 55154..55851 + 698 Protein_68 IS1 family transposase -
GTQ78_RS23005 55997..56254 + 258 WP_105278499.1 hypothetical protein -
GTQ78_RS23010 56291..57259 - 969 WP_000654811.1 IS5 family transposase -
GTQ78_RS23015 57294..57539 + 246 Protein_71 peptidoglycan DD-metalloendopeptidase family protein -
GTQ78_RS23020 57576..58280 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
GTQ78_RS23025 58369..59082 + 714 WP_001452812.1 DUF3363 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ant(3'')-Ia / sul3 / blaTEM-1B / floR / tet(A) / dfrA5 / qnrS13 - 1..113013 113013
- inside IScluster/Tn floR / tet(A) / dfrA5 / qnrS13 - 48688..80850 32162


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T146757 WP_000220560.1 NZ_CP047573:54798-55079 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T146757 NZ_CP062780:2315719-2315822 [Escherichia coli O157:H7]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT146757 WP_000121743.1 NZ_CP047573:54557-54808 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT146757 NZ_CP062780:c2315860-2315589 [Escherichia coli O157:H7]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References