Detailed information of TA system TA146747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3890571..3891147
• Replicon: chromosome
• Accession: NZ_CP047571
• Organism: Escherichia coli strain 2EC1

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: GTQ78_RS18840
• Protein ID: WP_160177639.1
• Coordinates: 3890860..3891147 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A829G8Z0
• Locus tag: GTQ78_RS18835
• Protein ID: WP_000063156.1
• Coordinates: 3890571..3890873 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTQ78_RS18820	3887167..3889317	+	2151	WP_001736090.1	pyruvate/proton symporter BtsT	-
GTQ78_RS18825	3889367..3889570	+	204	WP_000467859.1	YbdD/YjiX family protein	-
GTQ78_RS18830	3889581..3890537	+	957	WP_001297640.1	GTPase	-
GTQ78_RS18835	3890571..3890873	-	303	WP_000063156.1	BrnA antitoxin family protein	Antitoxin
GTQ78_RS18840	3890860..3891147	-	288	WP_160177639.1	BrnT family toxin	Toxin
GTQ78_RS18845	3891346..3892043	-	698	Protein_3684	IS1 family transposase	-
GTQ78_RS18850	3892100..3892195	+	96	Protein_3685	dienelactone hydrolase family protein	-
GTQ78_RS18855	3892341..3893573	+	1233	WP_001339512.1	multidrug efflux MFS transporter MdtM	-
GTQ78_RS18860	3893614..3894894	+	1281	WP_160177640.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3891346..3891639	293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11364.88 Da
• Isoelectric Point: 7.4024

>T146747 WP_160177639.1 NZ_CP047571:c3891147-3890860 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFERGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T146747 NZ_CP062779:69651-69809 [Escherichia coli O157:H7]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATAGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11521.99 Da
• Isoelectric Point: 9.4646

>AT146747 WP_000063156.1 NZ_CP047571:c3890873-3890571 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT146747 NZ_CP062779:69388-69586 [Escherichia coli O157:H7]
TCACACGGATTGCCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAATGTGGTCAGCGTGCTGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin

• AlphaFold DB: A0A829G8Z0