Detailed information of TA system TA146744

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3528517..3529196
• Replicon: chromosome
• Accession: NZ_CP047571
• Organism: Escherichia coli strain 2EC1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: GTQ78_RS17085
• Protein ID: WP_000854680.1
• Coordinates: 3528855..3529196 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: GTQ78_RS17080
• Protein ID: WP_000070396.1
• Coordinates: 3528517..3528834 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTQ78_RS17035	3523955..3524776	+	822	WP_000197388.1	DUF945 domain-containing protein	-
GTQ78_RS17040	3524993..3525694	+	702	WP_160177604.1	WYL domain-containing protein	-
GTQ78_RS17045	3525735..3525971	+	237	WP_001144031.1	hypothetical protein	-
GTQ78_RS17050	3525971..3526414	+	444	WP_000649865.1	hypothetical protein	-
GTQ78_RS17055	3526437..3526904	+	468	WP_001385283.1	hypothetical protein	-
GTQ78_RS17060	3526981..3527220	+	240	Protein_3339	DUF905 domain-containing protein	-
GTQ78_RS17065	3527318..3527776	+	459	WP_000211838.1	antirestriction protein	-
GTQ78_RS17070	3527792..3528268	+	477	WP_160177606.1	RadC family protein	-
GTQ78_RS17075	3528277..3528498	+	222	WP_000691994.1	DUF987 domain-containing protein	-
GTQ78_RS17080	3528517..3528834	+	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
GTQ78_RS17085	3528855..3529196	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
GTQ78_RS17090	3529862..3530071	+	210	Protein_3345	transposase	-
GTQ78_RS17095	3530113..3530856	-	744	WP_137541354.1	AraC family transcriptional regulator	-
GTQ78_RS17100	3531680..3532453	+	774	WP_000355482.1	hypothetical protein	-
GTQ78_RS17105	3532523..3533107	-	585	WP_001709920.1	tail fiber assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3529886..3530071	185

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T146744 WP_000854680.1 NZ_CP047571:3528855-3529196 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T146744 NZ_CP062778:c5081287-5081114 [Escherichia coli O157:H7]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT146744 WP_000070396.1 NZ_CP047571:3528517-3528834 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT146744 NZ_CP062778:c5081611-5081315 [Escherichia coli O157:H7]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7