Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3338482..3339100 Replicon chromosome
Accession NZ_CP047571
Organism Escherichia coli strain 2EC1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GTQ78_RS16135 Protein ID WP_001291435.1
Coordinates 3338882..3339100 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GTQ78_RS16130 Protein ID WP_000344800.1
Coordinates 3338482..3338856 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTQ78_RS16120 3333571..3334764 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GTQ78_RS16125 3334787..3337936 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
GTQ78_RS16130 3338482..3338856 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GTQ78_RS16135 3338882..3339100 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GTQ78_RS16140 3339272..3339823 + 552 WP_000102564.1 maltose O-acetyltransferase -
GTQ78_RS16145 3339939..3340409 + 471 WP_000136192.1 YlaC family protein -
GTQ78_RS16150 3340573..3342123 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GTQ78_RS16155 3342165..3342518 - 354 WP_000878140.1 DUF1428 domain-containing protein -
GTQ78_RS16165 3342897..3343208 + 312 WP_000409911.1 MGMT family protein -
GTQ78_RS16170 3343239..3343811 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T146743 WP_001291435.1 NZ_CP047571:3338882-3339100 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T146743 NZ_CP062778:c4984474-4984124 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT146743 WP_000344800.1 NZ_CP047571:3338482-3338856 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT146743 NZ_CP062778:c4984719-4984468 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References