Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2319031..2319669 Replicon chromosome
Accession NZ_CP047571
Organism Escherichia coli strain 2EC1

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag GTQ78_RS11140 Protein ID WP_000813794.1
Coordinates 2319493..2319669 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag GTQ78_RS11135 Protein ID WP_001270286.1
Coordinates 2319031..2319447 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTQ78_RS11115 2314183..2315124 - 942 WP_001251315.1 ABC transporter permease -
GTQ78_RS11120 2315125..2316138 - 1014 WP_160177460.1 ABC transporter ATP-binding protein -
GTQ78_RS11125 2316156..2317301 - 1146 WP_000047418.1 ABC transporter substrate-binding protein -
GTQ78_RS11130 2317546..2318952 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
GTQ78_RS11135 2319031..2319447 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
GTQ78_RS11140 2319493..2319669 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
GTQ78_RS11145 2319891..2320121 + 231 WP_160177461.1 YncJ family protein -
GTQ78_RS11150 2320213..2322174 - 1962 WP_160177674.1 23S rRNA 5-hydroxycytidine C2501 synthase -
GTQ78_RS11155 2322247..2322783 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
GTQ78_RS11160 2322836..2324050 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T146742 WP_000813794.1 NZ_CP047571:c2319669-2319493 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T146742 NZ_CP062778:4849589-4849930 [Escherichia coli O157:H7]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT146742 WP_001270286.1 NZ_CP047571:c2319447-2319031 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT146742 NZ_CP062778:c4849594-4849518 [Escherichia coli O157:H7]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References