Detailed information of TA system TA146725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4068168..4068764
• Replicon: chromosome
• Accession: NZ_CP047570
• Organism: Enterobacter hormaechei strain F2

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A837F9W8
• Locus tag: GTQ93_RS19375
• Protein ID: WP_023315213.1
• Coordinates: 4068168..4068470 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: GTQ93_RS19380
• Protein ID: WP_032672198.1
• Coordinates: 4068477..4068764 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTQ93_RS19355	4064387..4065736	+	1350	WP_006810501.1	lysine-sensitive aspartokinase 3	-
GTQ93_RS19360	4065830..4066774	+	945	WP_023295757.1	ketopantoate/pantoate/pantothenate transporter PanS	-
GTQ93_RS19365	4066825..4067097	+	273	WP_032668823.1	DUF3811 domain-containing protein	-
GTQ93_RS19370	4067098..4067970	-	873	WP_003860347.1	23S rRNA pseudouridine(2604) synthase RluF	-
GTQ93_RS19375	4068168..4068470	+	303	WP_023315213.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GTQ93_RS19380	4068477..4068764	+	288	WP_032672198.1	putative addiction module antidote protein	Antitoxin
GTQ93_RS19385	4068761..4070392	-	1632	WP_003860350.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11427.21 Da
• Isoelectric Point: 10.1042

>T146725 WP_023315213.1 NZ_CP047570:4068168-4068470 [Enterobacter hormaechei]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRVYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T146725 NZ_CP062778:739582-739896 [Escherichia coli O157:H7]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTTTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10088.51 Da
• Isoelectric Point: 4.7045

>AT146725 WP_032672198.1 NZ_CP047570:4068477-4068764 [Enterobacter hormaechei]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAEALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Nucleotide

• Length: 288 bp

>AT146725 NZ_CP062778:739893-740309 [Escherichia coli O157:H7]
ATGATTGCGATTGCCGACATCTTGCATGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACATCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A837F9W8