Detailed information of TA system TA146721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2070893..2071632
• Replicon: chromosome
• Accession: NZ_CP047570
• Organism: Enterobacter hormaechei strain F2

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: GTQ93_RS09890
• Protein ID: WP_160176976.1
• Coordinates: 2071147..2071632 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: GTQ93_RS09885
• Protein ID: WP_003857131.1
• Coordinates: 2070893..2071159 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTQ93_RS09865	2066375..2067361	+	987	WP_033487777.1	DUF979 domain-containing protein	-
GTQ93_RS09870	2067371..2068366	+	996	WP_033487776.1	DUF2891 domain-containing protein	-
GTQ93_RS09875	2068388..2069770	-	1383	WP_033487775.1	MFS transporter	-
GTQ93_RS09880	2069900..2070829	+	930	WP_063847896.1	LysR family transcriptional regulator	-
GTQ93_RS09885	2070893..2071159	+	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
GTQ93_RS09890	2071147..2071632	+	486	WP_160176976.1	GNAT family N-acetyltransferase	Toxin
GTQ93_RS09895	2071683..2072252	-	570	WP_063847895.1	hypothetical protein	-
GTQ93_RS09900	2072268..2073233	-	966	WP_058689390.1	hypothetical protein	-
GTQ93_RS09905	2073488..2073804	+	317	Protein_1937	ISNCY family transposase	-
GTQ93_RS09910	2074233..2074679	+	447	WP_014069654.1	DUF3290 domain-containing protein	-
GTQ93_RS09915	2074689..2075324	+	636	WP_015570517.1	DUF421 domain-containing protein	-
GTQ93_RS09920	2075508..2076122	-	615	WP_080337332.1	NUDIX hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17558.31 Da
• Isoelectric Point: 10.1788

>T146721 WP_160176976.1 NZ_CP047570:2071147-2071632 [Enterobacter hormaechei]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAMMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
K

Nucleotide

• Length: 486 bp

>T146721 NZ_CP062778:396397-396924 [Escherichia coli O157:H7]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT146721 WP_003857131.1 NZ_CP047570:2070893-2071159 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT146721 NZ_CP062778:396124-396390 [Escherichia coli O157:H7]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Antitoxin

• AlphaFold DB: A0A837FCR9