Detailed information of TA system TA146711

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 4506519..4508191
• Replicon: chromosome
• Accession: NZ_CP047569
• Organism: Microbulbifer sp. ALW1

Toxin (Protein)

• Gene name: HipA3
• Uniprot ID: -
• Locus tag: GRX76_RS18555
• Protein ID: WP_160154644.1
• Coordinates: 4506860..4508191 (+)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: HipB3
• Uniprot ID: -
• Locus tag: GRX76_RS18550
• Protein ID: WP_160154643.1
• Coordinates: 4506519..4506866 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GRX76_RS18530	4501924..4503303	-	1380	WP_160154639.1	alanine:cation symporter family protein	-
GRX76_RS18535	4503380..4504039	-	660	WP_160154640.1	helix-turn-helix domain-containing protein	-
GRX76_RS18540	4504083..4504364	-	282	WP_160154641.1	hypothetical protein	-
GRX76_RS18545	4504586..4506085	-	1500	WP_201277011.1	MFS transporter	-
GRX76_RS18550	4506519..4506866	+	348	WP_160154643.1	helix-turn-helix domain-containing protein	Antitoxin
GRX76_RS18555	4506860..4508191	+	1332	WP_160154644.1	type II toxin-antitoxin system HipA family toxin	Toxin
GRX76_RS18560	4508378..4508899	+	522	WP_160154645.1	hypothetical protein	-
GRX76_RS18565	4509167..4509616	+	450	WP_160154646.1	hypothetical protein	-
GRX76_RS18570	4509675..4510073	+	399	WP_160154647.1	hypothetical protein	-
GRX76_RS18575	4510106..4510801	-	696	WP_160154648.1	hypothetical protein	-
GRX76_RS18580	4510967..4511137	+	171	WP_160154649.1	DUF2986 domain-containing protein	-
GRX76_RS18585	4511205..4511495	+	291	WP_160154650.1	hypothetical protein	-
GRX76_RS18590	4511529..4512176	+	648	WP_160154651.1	hypothetical protein	-
GRX76_RS18595	4512272..4512643	+	372	WP_160154652.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (179-417)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-67)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 49916.43 Da
• Isoelectric Point: 5.8603

>T146711 WP_160154644.1 NZ_CP047569:4506860-4508191 [Microbulbifer sp. ALW1]
MVMEVIGVDYLGQDGQEQTAGAVSFDTDTGVGAFEYDPAFIDTGIELSPLKMPLARRIYSFPELRFDTFRGLPGLIADSL
PDDFGNAVLNAWMAAQGKHPQDISPLQRLQYTGRRGMGALEYTPATRIKKLNASQDIAIDELVAIAQEVLDHHSSFNVHL
DKTGEDNREAMMALMSVGMSAGGARPKAVLAFNKDFTHVRSGQTDVPEGFTHYLMKFDGVSEHNKDRETFGDPMGFGAME
YVYHQMASNCGIEMMPCHLLNEGNRRHFVTERFDRRDNNKVHIQTLNGIAHVDYKVPGSFSYAELFGIARQLKLSAKEAE
QLLIRMVFNIVARNHDDHAKNFAFQLNGKTWQLAPAYDLAYSYKPGSRWVNSHWMSLNGKRENFTREDIYSLEKVSPLFS
RRKIDSIVDNVVEQVSQWSALALENEVPESLIEEIRANLRLNI

Nucleotide

• Length: 1332 bp

>T146711 NZ_CP062774:c4945487-4945137 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 12598.28 Da
• Isoelectric Point: 5.9052

>AT146711 WP_160154643.1 NZ_CP047569:4506519-4506866 [Microbulbifer sp. ALW1]
MDPELKSPAAIAESLGQRLKQARLNRDLTQLEVAEHAGVSRKAVLNAEKGKVQLEVLVAILQALDLTAQLDNFLPPQPPS
PIQLAKLQGKKRQRASGQRGKNEGGDDDTQEEPEW

Nucleotide

• Length: 348 bp

>AT146711 NZ_CP062774:c4945732-4945481 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA