Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 22296..23919 Replicon plasmid unnamed
Accession NZ_CP047554
Organism Salmonella enterica subsp. enterica serovar Typhimurium strain SJTUF10231

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag DXN27_RS24600 Protein ID WP_000088046.1
Coordinates 22594..23919 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag DXN27_RS24595 Protein ID WP_024136327.1
Coordinates 22296..22589 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DXN27_RS24570 (DXN27_024500) 18168..19409 + 1242 WP_001053338.1 VWA domain-containing protein -
DXN27_RS25640 19500..19631 + 132 WP_000374059.1 hypothetical protein -
DXN27_RS24575 (DXN27_024505) 19972..20253 - 282 WP_000077926.1 hypothetical protein -
DXN27_RS24580 (DXN27_024510) 20303..20494 - 192 WP_000398480.1 hypothetical protein -
DXN27_RS24585 (DXN27_024515) 20586..20927 - 342 WP_001151575.1 hypothetical protein -
DXN27_RS24590 (DXN27_024520) 21300..21692 + 393 WP_000341066.1 hypothetical protein -
DXN27_RS24595 (DXN27_024525) 22296..22589 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
DXN27_RS24600 (DXN27_024530) 22594..23919 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
DXN27_RS24605 (DXN27_024535) 23980..24186 + 207 WP_000134171.1 hypothetical protein -
DXN27_RS24610 (DXN27_024540) 24288..24698 + 411 WP_000985911.1 hypothetical protein -
DXN27_RS24615 (DXN27_024545) 24711..25526 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
DXN27_RS24620 (DXN27_024550) 25780..26205 + 426 WP_001043843.1 hypothetical protein -
DXN27_RS24625 (DXN27_024555) 26659..26895 + 237 Protein_31 type I restriction-modification system subunit M N-terminal domain-containing protein -
DXN27_RS24630 (DXN27_024560) 26950..27249 + 300 WP_001572440.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / oqxA / oqxB - 1..213570 213570


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T146686 WP_000088046.1 NZ_CP047554:22594-23919 [Salmonella enterica subsp. enterica serovar Typhimurium]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T146686 NZ_CP062773:c2388-1348 [Escherichia coli O157:H7]
ATGGCACAGAACCCGTTTAAAGCACTAAATATCAATATTGACAAGATTGAGTCTGCTCTGACGCAGAATGGCGTCACAAA
CTATTCCTCTAATGTAAAAAACGAAAGAGAAACTCACATATCTGGCACATATAAAGGAATAGACTTCTTAATAAAACTAA
TGCCATCAGGCGGAAATACCACTATCGGAAGAGCGTCTGGGCAAAATAACACTTACTTTGATGAAATCGCCTTGATTATA
AAAGAAAACTGTTTGTATTCAGACACAAAGAACTTTGAATACACCATTCCAAAATTCAGTGATGATGACAGGGCAAATCT
ATTTGAATTTCTTTCTGAAGAGGGGATAACAATAACAGAAGATAATAATAACGATCCTAATTGTAAACACCAATATATTA
TGACCACCAGCAATGGTGACAGGGTCAGGGCAAAAATTTACAAGCGCGGCTCTATTCAATTCCAAGGAAAATACCTTCAA
ATCGCGAGTTTGATTAACGATTTCATGTGCTCAATACTAAACATGAAAGAGATTGTCGAACAAAAAAATAAAGAATTTAA
TGTTGACATAAAAAAAGAAACTATTGAGTCCGAGTTGCATAGCAAACTACCAAAAAGCATCGATAAAATCCATGAAGATA
TCAAAAAACAGCTATCATGCTCGCTAATAATGAAAAAAATAGATGTCGAAATGGAAGATTACTCAACATACTGCTTCTCT
GCATTAAGAGCCATAGAAGGCTTTATATATCAAATACTTAATGATGTTTGCAATCCATCATCATCAAAGAACCTTGGCGA
ATACTTCACTGAAAACAAACCCAAATATATAATCAGAGAAATACACCAAGAAACTATAAATGGTGAAATAGCGGAAGTTT
TGTGTGAATGCTACACTTACTGGCATGAGAACAGGCATGGTTTGTTTCATATGAAACCAGGAATAGCTGACACGAAGACA
ATTAACAAATTAGAATCAATCGCAATCATCGATACCGTTTGCCAATTAATAGATGGTGGCGTAGCTAGGTTGAAATTATG
A

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT146686 WP_024136327.1 NZ_CP047554:22296-22589 [Salmonella enterica subsp. enterica serovar Typhimurium]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT146686 NZ_CP062773:c1351-971 [Escherichia coli O157:H7]
ATGAAAAAAGATAAAAAATATCAAATAGAAGCAATAAAAAATAAAGATAAAACTTTATTTATTGTCTATGCTACTGATAT
TTATAGCCCGAGCGAATTTTTCTCAAAAATCGAATCCGACTTGAAGAAAAAGAAAAGCAAGGGTGATGTTTTTTTTGATT
TAATAATTCCTAACGGTGGAAAAAAAGATCGTTACGTCTATACGTCATTTAATGGCGAGAAGTTTTCAAGTTACACATTA
AACAAAGTTACGAAAACTGATGAATATAATGATTTATCTGAGCTCTCGGCTTCGTTCTTTAAAAAAAACTTTGATAAGAT
CAACGTAAACCTTCTATCCAAAGCCACATCATTTGCTTTGAAAAAAGGCATTCCAATATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References