Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 117800..119423 Replicon plasmid unnamed
Accession NZ_CP047547
Organism Salmonella enterica subsp. enterica serovar Typhimurium strain SJTUF10236

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag DXN28_RS25130 Protein ID WP_000088046.1
Coordinates 117800..119125 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag DXN28_RS25135 Protein ID WP_024136327.1
Coordinates 119130..119423 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DXN28_RS25100 (DXN28_025015) 114470..114769 - 300 WP_001572440.1 hypothetical protein -
DXN28_RS25105 (DXN28_025020) 114824..115060 - 237 Protein_129 type I restriction-modification system subunit M N-terminal domain-containing protein -
DXN28_RS25110 (DXN28_025025) 115514..115939 - 426 WP_001043843.1 hypothetical protein -
DXN28_RS25115 (DXN28_025030) 116193..117008 - 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
DXN28_RS25120 (DXN28_025035) 117021..117431 - 411 WP_000985911.1 hypothetical protein -
DXN28_RS25125 (DXN28_025040) 117533..117739 - 207 WP_000134171.1 hypothetical protein -
DXN28_RS25130 (DXN28_025045) 117800..119125 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
DXN28_RS25135 (DXN28_025050) 119130..119423 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
DXN28_RS25140 (DXN28_025055) 120027..120419 - 393 WP_000341066.1 hypothetical protein -
DXN28_RS25145 (DXN28_025060) 120792..121133 + 342 WP_001151575.1 hypothetical protein -
DXN28_RS25150 (DXN28_025065) 121225..121416 + 192 WP_000398480.1 hypothetical protein -
DXN28_RS25155 (DXN28_025070) 121466..121747 + 282 WP_000077926.1 hypothetical protein -
DXN28_RS25850 122088..122219 - 132 WP_000374059.1 hypothetical protein -
DXN28_RS25160 (DXN28_025075) 122310..123551 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid oqxB / oqxA / sul1 / dfrA12 / aph(3')-Ia / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / floR / sul2 / aph(4)-Ia / aac(3)-IVa / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE - 1..250477 250477


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T146611 WP_000088046.1 NZ_CP047547:c119125-117800 [Salmonella enterica subsp. enterica serovar Typhimurium]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T146611 NZ_CP062766:c3053428-3053273 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT146611 WP_024136327.1 NZ_CP047547:c119423-119130 [Salmonella enterica subsp. enterica serovar Typhimurium]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT146611 NZ_CP062766:3053440-3053498 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References