Detailed information of TA system TA146562

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 206695..207455
• Replicon: chromosome
• Accession: NZ_CP047544
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain SJTUF10250

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q57IH1
• Locus tag: DXN29_RS00965
• Protein ID: WP_000533909.1
• Coordinates: 206970..207455 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: M7RHS4
• Locus tag: DXN29_RS00960
• Protein ID: WP_000965886.1
• Coordinates: 206695..206982 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXN29_RS00935 (201990)	201990..202292	+	303	WP_000605590.1	YsaB family lipoprotein	-
DXN29_RS00940 (202431)	202431..203342	+	912	WP_001168551.1	glycine--tRNA ligase subunit alpha	-
DXN29_RS00945 (203352)	203352..205421	+	2070	WP_001291736.1	glycine--tRNA ligase subunit beta	-
DXN29_RS00950 (205603)	205603..205878	+	276	Protein_189	IS3 family transposase	-
DXN29_RS00955 (206050)	206050..206517	+	468	WP_000702452.1	GNAT family N-acetyltransferase	-
DXN29_RS00960 (206695)	206695..206982	+	288	WP_000965886.1	DUF1778 domain-containing protein	Antitoxin
DXN29_RS00965 (206970)	206970..207455	+	486	WP_000533909.1	GNAT family N-acetyltransferase	Toxin
DXN29_RS00970 (207827)	207827..208366	-	540	WP_000047149.1	copper-binding periplasmic metallochaperone CueP	-
DXN29_RS00975 (208540)	208540..208752	-	213	WP_000014594.1	RNA chaperone/antiterminator CspA	-
DXN29_RS00980 (209041)	209041..209331	-	291	WP_000455790.1	HTH-type transcriptional regulator	-
DXN29_RS00985 (209770)	209770..210480	+	711	WP_000190524.1	DUF3053 domain-containing protein	-
DXN29_RS00990 (210530)	210530..211504	-	975	WP_000804674.1	glyoxylate/hydroxypyruvate reductase GhrB	-
DXN29_RS00995 (211723)	211723..212385	-	663	WP_000747548.1	OmpA family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (31-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-94)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17703.41 Da
• Isoelectric Point: 9.8719

>T146562 WP_000533909.1 NZ_CP047544:206970-207455 [Salmonella enterica subsp. enterica serovar Typhimurium]
VGRVTAPEPLSAFHQVAEFVSGEAVLDDWLKQKGLKNQALGAARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFHGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKSSQTQQRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T146562 NZ_CP062761:4494310-4494570 [Escherichia coli O157:H7]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10950.60 Da
• Isoelectric Point: 4.6811

>AT146562 WP_000965886.1 NZ_CP047544:206695-206982 [Salmonella enterica subsp. enterica serovar Typhimurium]
MLYKGCLMKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILETACQAAEKVILDRRVFNFNDEQYEEFINLLDAPVADD
PVIEKLLARKPQWDV

Nucleotide

• Length: 288 bp

>AT146562 NZ_CP062761:4494031-4494291 [Escherichia coli O157:H7]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Structures

Toxin

• PDB: 7F36
• PDB: 7AK8
• PDB: 5FVJ

Antitoxin

• AlphaFold DB: A0A3V2JDX2