Detailed information of TA system TA146561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 204103..205726
• Replicon: plasmid unnamed
• Accession: NZ_CP047543
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain SJTUF10330

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: DXN30_RS25535
• Protein ID: WP_084568131.1
• Coordinates: 204401..205726 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: DXN30_RS25530
• Protein ID: WP_024136327.1
• Coordinates: 204103..204396 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXN30_RS25505 (DXN30_025415)	199975..201216	+	1242	WP_001053338.1	VWA domain-containing protein	-
DXN30_RS25665	201307..201438	+	132	WP_000374059.1	hypothetical protein	-
DXN30_RS25510 (DXN30_025420)	201779..202060	-	282	WP_000077926.1	hypothetical protein	-
DXN30_RS25515 (DXN30_025425)	202110..202301	-	192	WP_000398480.1	hypothetical protein	-
DXN30_RS25520 (DXN30_025430)	202393..202734	-	342	WP_001151575.1	hypothetical protein	-
DXN30_RS25525 (DXN30_025435)	203107..203499	+	393	WP_000341066.1	hypothetical protein	-
DXN30_RS25530 (DXN30_025440)	204103..204396	+	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
DXN30_RS25535 (DXN30_025445)	204401..205726	+	1326	WP_084568131.1	type II toxin-antitoxin system HipA family toxin	Toxin
DXN30_RS25540 (DXN30_025450)	205787..205993	+	207	WP_000134171.1	hypothetical protein	-
DXN30_RS25545 (DXN30_025455)	206095..206505	+	411	WP_000985911.1	hypothetical protein	-
DXN30_RS25550 (DXN30_025460)	206518..207333	+	816	WP_001572439.1	HNH endonuclease signature motif containing protein	-
DXN30_RS25555 (DXN30_025465)	207587..208012	+	426	WP_001043843.1	hypothetical protein	-
DXN30_RS25560 (DXN30_025470)	208466..208702	+	237	Protein_217	type I restriction-modification system subunit M N-terminal domain-containing protein	-
DXN30_RS25565 (DXN30_025475)	208757..209056	+	300	WP_001572440.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / aac(3)-IVa / aph(4)-Ia / sul2 / floR	-	1..216197	216197

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (155-404)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50259.23 Da
• Isoelectric Point: 6.1927

>T146561 WP_084568131.1 NZ_CP047543:204401-205726 [Salmonella enterica subsp. enterica serovar Typhimurium]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTEPEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T146561 NZ_CP062761:c4488311-4487913 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT146561 WP_024136327.1 NZ_CP047543:204103-204396 [Salmonella enterica subsp. enterica serovar Typhimurium]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

>AT146561 NZ_CP062761:c4488607-4488314 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A5Z6V2D1