Detailed information of TA system TA146481

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 103296..104919
• Replicon: plasmid unnamed
• Accession: NZ_CP047536
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain SJTUF10452

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: DXN08_RS23920
• Protein ID: WP_084568131.1
• Coordinates: 103594..104919 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: DXN08_RS23915
• Protein ID: WP_024136327.1
• Coordinates: 103296..103589 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXN08_RS23890 (DXN08_023785)	99168..100409	+	1242	WP_001053338.1	VWA domain-containing protein	-
DXN08_RS24805	100500..100631	+	132	WP_000374059.1	hypothetical protein	-
DXN08_RS23895 (DXN08_023790)	100972..101253	-	282	WP_000077926.1	hypothetical protein	-
DXN08_RS23900 (DXN08_023795)	101303..101494	-	192	WP_000398480.1	hypothetical protein	-
DXN08_RS23905 (DXN08_023800)	101586..101927	-	342	WP_001151575.1	hypothetical protein	-
DXN08_RS23910 (DXN08_023805)	102300..102692	+	393	WP_000341066.1	hypothetical protein	-
DXN08_RS23915 (DXN08_023810)	103296..103589	+	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
DXN08_RS23920 (DXN08_023815)	103594..104919	+	1326	WP_084568131.1	type II toxin-antitoxin system HipA family toxin	Toxin
DXN08_RS23925 (DXN08_023820)	104980..105186	+	207	WP_000134171.1	hypothetical protein	-
DXN08_RS23930 (DXN08_023825)	105288..105698	+	411	WP_000985911.1	hypothetical protein	-
DXN08_RS23935 (DXN08_023830)	105711..106526	+	816	WP_001572439.1	HNH endonuclease signature motif containing protein	-
DXN08_RS23940 (DXN08_023835)	106780..107205	+	426	WP_001043843.1	hypothetical protein	-
DXN08_RS23945 (DXN08_023840)	107659..107895	+	237	Protein_101	type I restriction-modification system subunit M N-terminal domain-containing protein	-
DXN08_RS23950 (DXN08_023845)	107950..108249	+	300	WP_001572440.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / aac(3)-IVa / aph(4)-Ia / sul2 / floR / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3')-Ia / dfrA12	-	1..243126	243126

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (155-404)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50259.23 Da
• Isoelectric Point: 6.1927

>T146481 WP_084568131.1 NZ_CP047536:103594-104919 [Salmonella enterica subsp. enterica serovar Typhimurium]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTEPEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T146481 NZ_CP062755:283379-283486 [Escherichia coli O157:H7]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT146481 WP_024136327.1 NZ_CP047536:103296-103589 [Salmonella enterica subsp. enterica serovar Typhimurium]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

>AT146481 NZ_CP062755:c283331-283265 [Escherichia coli O157:H7]
GGTCTAGGTTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Antitoxin

• AlphaFold DB: A0A5Z6V2D1