Detailed information of TA system TA146406

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 84084..85707
• Replicon: plasmid unnamed
• Accession: NZ_CP047528
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain SJTUF10648

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: DXN11_RS24865
• Protein ID: WP_084568131.1
• Coordinates: 84382..85707 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: DXN11_RS24860
• Protein ID: WP_024136327.1
• Coordinates: 84084..84377 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXN11_RS24835 (DXN11_024760)	79956..81197	+	1242	WP_001053338.1	VWA domain-containing protein	-
DXN11_RS25850	81288..81419	+	132	WP_000374059.1	hypothetical protein	-
DXN11_RS24840 (DXN11_024765)	81760..82041	-	282	WP_000077926.1	hypothetical protein	-
DXN11_RS24845 (DXN11_024770)	82091..82282	-	192	WP_000398480.1	hypothetical protein	-
DXN11_RS24850 (DXN11_024775)	82374..82715	-	342	WP_001151575.1	hypothetical protein	-
DXN11_RS24855 (DXN11_024780)	83088..83480	+	393	WP_000341066.1	hypothetical protein	-
DXN11_RS24860 (DXN11_024785)	84084..84377	+	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
DXN11_RS24865 (DXN11_024790)	84382..85707	+	1326	WP_084568131.1	type II toxin-antitoxin system HipA family toxin	Toxin
DXN11_RS24870 (DXN11_024795)	85768..85974	+	207	WP_000134171.1	hypothetical protein	-
DXN11_RS24875 (DXN11_024800)	86076..86486	+	411	WP_000985911.1	hypothetical protein	-
DXN11_RS24880 (DXN11_024805)	86499..87314	+	816	WP_001572439.1	HNH endonuclease signature motif containing protein	-
DXN11_RS24885 (DXN11_024810)	87568..87993	+	426	WP_001043843.1	hypothetical protein	-
DXN11_RS24890 (DXN11_024815)	88447..88683	+	237	Protein_86	type I restriction-modification system subunit M N-terminal domain-containing protein	-
DXN11_RS24895 (DXN11_024820)	88738..89037	+	300	WP_001572440.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / aac(3)-IVa / aph(4)-Ia / sul2 / floR / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3')-Ia / dfrA12 / oqxA / oqxB	-	1..249152	249152

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (155-404)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50259.23 Da
• Isoelectric Point: 6.1927

>T146406 WP_084568131.1 NZ_CP047528:84382-85707 [Salmonella enterica subsp. enterica serovar Typhimurium]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTEPEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T146406 NZ_CP062746:c3393096-3392993 [Escherichia coli O157:H7]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT146406 WP_024136327.1 NZ_CP047528:84084-84377 [Salmonella enterica subsp. enterica serovar Typhimurium]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

>AT146406 NZ_CP062746:3392955-3393226 [Escherichia coli O157:H7]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A5Z6V2D1