Detailed information of TA system TA146325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4666584..4667137
• Replicon: chromosome
• Accession: NZ_CP047495
• Organism: Pectobacterium brasiliense strain 1692

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: GT391_RS20895
• Protein ID: WP_010287537.1
• Coordinates: 4666584..4666898 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: GT391_RS20900
• Protein ID: WP_010287535.1
• Coordinates: 4666901..4667137 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GT391_RS20870	4661763..4663625	+	1863	WP_159398234.1	type IV secretory system conjugative DNA transfer family protein	-
GT391_RS20875	4663634..4664380	+	747	WP_039320867.1	hypothetical protein	-
GT391_RS20880	4664786..4665253	+	468	WP_199559768.1	Lrp/AsnC family transcriptional regulator	-
GT391_RS20885	4665250..4666110	-	861	WP_010287540.1	EamA family transporter	-
GT391_RS20890	4666296..4666550	+	255	WP_039320862.1	hypothetical protein	-
GT391_RS20895	4666584..4666898	-	315	WP_010287537.1	CcdB family protein	Toxin
GT391_RS20900	4666901..4667137	-	237	WP_010287535.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
GT391_RS20905	4667457..4668401	-	945	WP_159398235.1	DUF1738 domain-containing protein	-
GT391_RS20910	4668787..4669509	+	723	WP_072014229.1	MurR/RpiR family transcriptional regulator	-
GT391_RS20915	4669687..4671114	+	1428	WP_010284508.1	glycoside hydrolase family 1 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4641211..4681021	39810

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11493.40 Da
• Isoelectric Point: 7.9859

>T146325 WP_010287537.1 NZ_CP047495:c4666898-4666584 [Pectobacterium brasiliense]
MQFTVYGNTGKSVVYPLLLDVTSDIIGQLNRRIAIPLLPIEKYPAGRRPDRLVPVVRLTDGKEYAVMTHELASIPVQALG
AVFCDASQYRSQVKAAIDFLIDGF

Nucleotide

• Length: 315 bp

>T146325 NZ_CP062739:c4920007-4919693 [Escherichia coli O157:H7]
ATGCAATTTACGGTATACCGCAGTCGCAGTAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8964.00 Da
• Isoelectric Point: 6.7173

>AT146325 WP_010287535.1 NZ_CP047495:c4667137-4666901 [Pectobacterium brasiliense]
MTAKQRNTQSVTMTVERALLVRARAAGINLSATLTTALDAELRHHEAKKWQEENREAIDALNRFHDEQGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT146325 NZ_CP062739:c4920243-4920010 [Escherichia coli O157:H7]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG