Detailed information of TA system TA146323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3376761..3377314
• Replicon: chromosome
• Accession: NZ_CP047495
• Organism: Pectobacterium brasiliense strain 1692

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: GT391_RS15050
• Protein ID: WP_010287131.1
• Coordinates: 3377000..3377314 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A5S4UGL1
• Locus tag: GT391_RS15045
• Protein ID: WP_010287128.1
• Coordinates: 3376761..3376997 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GT391_RS15035	3372173..3374533	-	2361	WP_010277930.1	sensor histidine kinase	-
GT391_RS15040	3375496..3376440	+	945	WP_159398225.1	DUF1738 domain-containing protein	-
GT391_RS15045	3376761..3376997	+	237	WP_010287128.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
GT391_RS15050	3377000..3377314	+	315	WP_010287131.1	CcdB family protein	Toxin
GT391_RS15055	3377671..3378672	-	1002	WP_010287133.1	hypothetical protein	-
GT391_RS15060	3379018..3379557	-	540	WP_010287135.1	hypothetical protein	-
GT391_RS15065	3379797..3380564	-	768	WP_010287139.1	nucleotidyltransferase domain-containing protein	-
GT391_RS15070	3380699..3381445	-	747	WP_010287890.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3364509..3406946	42437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11554.49 Da
• Isoelectric Point: 5.7681

>T146323 WP_010287131.1 NZ_CP047495:3377000-3377314 [Pectobacterium brasiliense]
MQFSVYGNTGKSVVYPLLLDVTSDIIGQLNRRIVIPLLPIEKYPAGRRPDRLVPVVMLTDDKEYAVMTHELASIPVQALG
AVFCDASQYRSQIKAAIDFLIDGF

Nucleotide

• Length: 315 bp

>T146323 NZ_CP062739:c4682294-4681896 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8998.01 Da
• Isoelectric Point: 5.6999

>AT146323 WP_010287128.1 NZ_CP047495:3376761-3376997 [Pectobacterium brasiliense]
MTAKQRNTQSVTMTVERALLVRAREAGINLSATLSTALDAELRRHEAKIWQEENREAIDALNRFHDENGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT146323 NZ_CP062739:c4682590-4682297 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A5S4UGL1