Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1829047..1829664 Replicon chromosome
Accession NZ_CP047495
Organism Pectobacterium brasiliense strain 1692

Toxin (Protein)


Gene name hicA Uniprot ID C6DHN3
Locus tag GT391_RS08155 Protein ID WP_010281507.1
Coordinates 1829485..1829664 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0M2EY98
Locus tag GT391_RS08150 Protein ID WP_010281508.1
Coordinates 1829047..1829457 (-) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GT391_RS08135 1824912..1825955 - 1044 WP_010281514.1 DNA-binding transcriptional regulator CytR -
GT391_RS08140 1826264..1828462 - 2199 WP_010281513.1 primosomal protein N' -
GT391_RS08145 1828735..1828950 + 216 WP_010281510.1 50S ribosomal protein L31 -
GT391_RS08150 1829047..1829457 - 411 WP_010281508.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GT391_RS08155 1829485..1829664 - 180 WP_010281507.1 type II toxin-antitoxin system HicA family toxin Toxin
GT391_RS08160 1829748..1830758 - 1011 WP_010281503.1 iron ABC transporter permease -
GT391_RS08165 1830755..1831717 - 963 WP_010281502.1 ABC transporter substrate-binding protein -
GT391_RS08170 1831727..1832524 - 798 WP_010281500.1 ABC transporter ATP-binding protein -
GT391_RS08175 1832758..1833075 - 318 WP_005973355.1 met regulon transcriptional regulator MetJ -
GT391_RS08180 1833263..1834423 + 1161 WP_010281494.1 cystathionine gamma-synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6560.69 Da        Isoelectric Point: 10.7838

>T146320 WP_010281507.1 NZ_CP047495:c1829664-1829485 [Pectobacterium brasiliense]
MDSRTLIAEIKADGWELIRVNGSHHHFMHPSKPGLVTIPHPKKDLPIGTVKSIRKQAGI

Download         Length: 180 bp

>T146320 NZ_CP062739:3793039-3793326 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 14492.40 Da        Isoelectric Point: 4.4493

>AT146320 WP_010281508.1 NZ_CP047495:c1829457-1829047 [Pectobacterium brasiliense]
MFYPIAIEAGDDTHAYGVTVPDLPGCFSAGDTLDDAIANAKEAITGHIELLIEMGQDVPTVSSVGKLAKSAEYAGYTWAV
VDIDVTRLMGGSEKINVTLPKSLIDRIDRCVASNPEFKSRSGFLAQAALERISSSR

Download         Length: 411 bp

>AT146320 NZ_CP062739:3792848-3793039 [Escherichia coli O157:H7]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W2I1S0

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2EY98

References