Detailed information of TA system TA146316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 1413793..1414394
• Replicon: chromosome
• Accession: NZ_CP047495
• Organism: Pectobacterium brasiliense strain 1692

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: GT391_RS06280
• Protein ID: WP_010284261.1
• Coordinates: 1414011..1414394 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A3S1A917
• Locus tag: GT391_RS06275
• Protein ID: WP_010284263.1
• Coordinates: 1413793..1414014 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GT391_RS06250	1408943..1409890	+	948	WP_010284276.1	isoaspartyl peptidase/L-asparaginase	-
GT391_RS06255	1410103..1411011	+	909	WP_010284274.1	LysR family transcriptional regulator	-
GT391_RS06260	1411111..1411974	+	864	WP_010284271.1	YicC family protein	-
GT391_RS06265	1412373..1413146	+	774	WP_039320466.1	hypothetical protein	-
GT391_RS06270	1413421..1413744	+	324	WP_010284265.1	hypothetical protein	-
GT391_RS06275	1413793..1414014	+	222	WP_010284263.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GT391_RS06280	1414011..1414394	+	384	WP_010284261.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
GT391_RS06285	1414558..1415115	+	558	WP_010284259.1	hypothetical protein	-
GT391_RS06290	1415158..1415703	-	546	WP_100068802.1	hypothetical protein	-
GT391_RS06295	1415835..1416221	+	387	WP_172644798.1	hypothetical protein	-
GT391_RS06300	1416253..1416615	+	363	WP_052051621.1	DUF596 domain-containing protein	-
GT391_RS06305	1416682..1417851	-	1170	WP_010284250.1	AI-2E family transporter	-
GT391_RS06310	1417945..1418445	-	501	WP_010284247.1	GrpB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (5-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.31 Da
• Isoelectric Point: 7.3172

>T146316 WP_010284261.1 NZ_CP047495:1414011-1414394 [Pectobacterium brasiliense]
MNWVSAQEVIAFHDRILRRLPGVTGMPDPGRAEAIIYRVQNRTHYEGITDIFELAATYWVAVARGHIFNDGNKRTAFFVT
MTFLYRNGVLVRDNDNTLENLTVEAATGAKNVDELAQQLRTRVQPLG

Nucleotide

• Length: 384 bp

>T146316 NZ_CP062739:c3065929-3065753 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8147.06 Da
• Isoelectric Point: 5.0359

>AT146316 WP_010284263.1 NZ_CP047495:1413793-1414014 [Pectobacterium brasiliense]
MRIYTSTQARANISEVLDAATHGEPVEITRRDGSSAVVISKAEFEAYQNAKLDAEFDLIMQRHGHTIEALTDR

Nucleotide

• Length: 222 bp

>AT146316 NZ_CP062739:c3065707-3065291 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCAGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A3S1A917