Detailed information of TA system TA146313

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 762093..762618
• Replicon: chromosome
• Accession: NZ_CP047495
• Organism: Pectobacterium brasiliense strain 1692

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GT391_RS03455
• Protein ID: WP_010282373.1
• Coordinates: 762334..762618 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C6D956
• Locus tag: GT391_RS03450
• Protein ID: WP_005973616.1
• Coordinates: 762093..762344 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GT391_RS03425	759082..759657	+	576	WP_010282383.1	hypothetical protein	-
GT391_RS03430	759782..760033	+	252	WP_010282381.1	prevent-host-death protein	-
GT391_RS21565	760158..760304	+	147	WP_167740034.1	hypothetical protein	-
GT391_RS03440	760584..760850	+	267	WP_010282378.1	DUF1778 domain-containing protein	-
GT391_RS03445	760852..761367	+	516	WP_010282375.1	GNAT family N-acetyltransferase	-
GT391_RS03450	762093..762344	+	252	WP_005973616.1	prevent-host-death protein	Antitoxin
GT391_RS03455	762334..762618	+	285	WP_010282373.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GT391_RS03460	762794..763378	-	585	WP_010282372.1	putative adenosine monophosphate-protein transferase Fic	-
GT391_RS03465	763368..763862	-	495	WP_010282369.1	helix-turn-helix domain-containing protein	-
GT391_RS03470	764423..765001	+	579	WP_010282353.1	hypothetical protein	-
GT391_RS03475	765368..766150	+	783	WP_010282351.1	transporter substrate-binding domain-containing protein	-
GT391_RS03480	766418..767044	+	627	WP_010282349.1	LysE family transporter	-
GT391_RS03485	767090..767572	-	483	WP_039274311.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10907.88 Da
• Isoelectric Point: 10.1988

>T146313 WP_010282373.1 NZ_CP047495:762334-762618 [Pectobacterium brasiliense]
MSYKLEFEEHALKEFKKLGAPVREQFTKKLKAVLQNPHVPANRLHGMADCYKIKLRSAGYRLVYQVLEHEIVVLVLAVGK
RERSEVYKAAIDRL

Nucleotide

• Length: 285 bp

>T146313 NZ_CP062739:c2256924-2256646 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9079.35 Da
• Isoelectric Point: 5.0807

>AT146313 WP_005973616.1 NZ_CP047495:762093-762344 [Pectobacterium brasiliense]
MSVQPILANAAVSISDFKKSPNAALKEANGEPVAVLTNGRISGYYVSPETWEALADYQEDIELAKIARSRMKGKRVKVDL
DEL

Nucleotide

• Length: 252 bp

>AT146313 NZ_CP062739:c2257117-2256926 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0G4JQP6