Detailed information of TA system TA146298

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1366199..1367115
• Replicon: chromosome
• Accession: NZ_CP047485
• Organism: Bacillus subtilis strain BJQ0005

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: O34853
• Locus tag: GTW28_RS07305
• Protein ID: WP_003244695.1
• Coordinates: 1366369..1367115 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: GTW28_RS07300
• Protein ID: WP_003232646.1
• Coordinates: 1366199..1366369 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTW28_RS07265	1363059..1363388	+	330	WP_046380959.1	XkdW family protein	-
GTW28_RS07270	1363385..1363549	+	165	WP_014479563.1	XkdX family protein	-
GTW28_RS07275	1363596..1364435	+	840	WP_046380960.1	phage-like element PBSX protein XepA	-
GTW28_RS07280	1364488..1364757	+	270	WP_015252265.1	hemolysin XhlA family protein	-
GTW28_RS07285	1364770..1365033	+	264	WP_003232653.1	phage holin	-
GTW28_RS07290	1365046..1365939	+	894	WP_003245230.1	N-acetylmuramoyl-L-alanine amidase	-
GTW28_RS07295	1365976..1366113	-	138	WP_003232648.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
GTW28_RS07300	1366199..1366369	-	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
GTW28_RS07305	1366369..1367115	-	747	WP_003244695.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
GTW28_RS07310	1367225..1368226	-	1002	WP_003232642.1	inorganic phosphate transporter	-
GTW28_RS07315	1368239..1368856	-	618	WP_003218470.1	DUF47 domain-containing protein	-
GTW28_RS07320	1369132..1370448	-	1317	WP_046380961.1	serine/threonine exchanger	-
GTW28_RS07325	1370837..1371787	+	951	WP_014479571.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1332172..1375496	43324

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29061.50 Da
• Isoelectric Point: 4.6191

>T146298 WP_003244695.1 NZ_CP047485:c1367115-1366369 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T146298 NZ_CP062737:2066-2341 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT146298 WP_003232646.1 NZ_CP047485:c1366369-1366199 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT146298 NZ_CP062737:1782-2066 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• PDB: 3O6Q

Antitoxin

• PDB: 3O6Q
• AlphaFold DB: G4EXD8