146297

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 509655..510291 Replicon chromosome
Accession NZ_CP047485
Organism Bacillus subtilis strain BJQ0005

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag GTW28_RS02625 Protein ID WP_003156187.1
Coordinates 509941..510291 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag GTW28_RS02620 Protein ID WP_003225183.1
Coordinates 509655..509936 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTW28_RS02600 506014..506613 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
GTW28_RS02605 506708..507073 + 366 WP_120363269.1 holo-ACP synthase -
GTW28_RS02610 507239..508255 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
GTW28_RS02615 508370..509539 + 1170 WP_015252766.1 alanine racemase -
GTW28_RS02620 509655..509936 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
GTW28_RS02625 509941..510291 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GTW28_RS02630 510406..511230 + 825 WP_080320324.1 RsbT co-antagonist protein RsbRA -
GTW28_RS02635 511235..511600 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
GTW28_RS02640 511604..512005 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
GTW28_RS02645 512017..513024 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
GTW28_RS02650 513086..513415 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
GTW28_RS02655 513412..513894 + 483 WP_003234299.1 anti-sigma B factor RsbW -
GTW28_RS02660 513860..514648 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
GTW28_RS02665 514648..515247 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T146297 WP_003156187.1 NZ_CP047485:509941-510291 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T146297 NZ_CP062736:c5054008-5053835 [Escherichia coli O157:H7]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT146297 WP_003225183.1 NZ_CP047485:509655-509936 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT146297 NZ_CP062736:c5054332-5054036 [Escherichia coli O157:H7]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB G4NU32

References