Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 54414..55057 Replicon plasmid pDY612-4
Accession NZ_CP047473
Organism Escherichia coli strain DY612

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag GTK51_RS23495 Protein ID WP_001044768.1
Coordinates 54414..54830 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag GTK51_RS23500 Protein ID WP_001261287.1
Coordinates 54827..55057 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTK51_RS23480 (GTK51_23195) 50913..51503 - 591 WP_000194575.1 hypothetical protein -
GTK51_RS23485 (GTK51_23200) 51503..51760 - 258 WP_000343085.1 hypothetical protein -
GTK51_RS23490 (GTK51_23205) 52114..54252 + 2139 WP_000350638.1 AAA family ATPase -
GTK51_RS23495 (GTK51_23210) 54414..54830 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
GTK51_RS23500 (GTK51_23215) 54827..55057 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
GTK51_RS23505 (GTK51_23220) 55353..55625 + 273 WP_061602529.1 hypothetical protein -
GTK51_RS23510 (GTK51_23225) 55672..56376 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
GTK51_RS23515 (GTK51_23230) 56602..57462 + 861 WP_278182359.1 aminoglycoside N-acetyltransferase AAC(3)-IId -
GTK51_RS23520 (GTK51_23235) 57475..58017 + 543 WP_000587837.1 AAA family ATPase -
GTK51_RS23525 (GTK51_23240) 58499..58690 - 192 WP_000951934.1 hypothetical protein -
GTK51_RS23530 (GTK51_23245) 58714..58941 + 228 WP_000248278.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul2 / tet(A) / aph(3'')-Ib / aph(6)-Id / aac(3)-IId / blaTEM-1B / aph(3')-Ia / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / tet(X4) - 1..97200 97200


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T146296 WP_001044768.1 NZ_CP047473:c54830-54414 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T146296 NZ_CP062736:c4957194-4956844 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT146296 WP_001261287.1 NZ_CP047473:c55057-54827 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT146296 NZ_CP062736:c4957439-4957188 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References