Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 18525..19072 Replicon plasmid pDY612-4
Accession NZ_CP047473
Organism Escherichia coli strain DY612

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag GTK51_RS23290 Protein ID WP_001384452.1
Coordinates 18525..18803 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A1M1SX19
Locus tag GTK51_RS23295 Protein ID WP_021546940.1
Coordinates 18803..19072 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTK51_RS23265 (GTK51_22985) 14514..15398 - 885 WP_000058717.1 EamA family transporter -
GTK51_RS23270 (GTK51_22990) 15536..15928 - 393 WP_001351729.1 isochorismatase family cysteine hydrolase -
GTK51_RS23275 (GTK51_22995) 15932..17683 + 1752 Protein_20 Tn3-like element TnAs1 family transposase -
GTK51_RS23280 (GTK51_23000) 17936..18172 - 237 WP_223417154.1 transposase -
GTK51_RS23285 (GTK51_23005) 18267..18479 - 213 Protein_22 3'-5' exonuclease -
GTK51_RS23290 (GTK51_23010) 18525..18803 - 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
GTK51_RS23295 (GTK51_23015) 18803..19072 - 270 WP_021546940.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
GTK51_RS23300 (GTK51_23020) 19759..20879 + 1121 WP_094316150.1 IS3-like element ISSen4 family transposase -
GTK51_RS23305 (GTK51_23025) 20880..21218 + 339 WP_063116667.1 hypothetical protein -
GTK51_RS23310 (GTK51_23030) 21266..22255 - 990 WP_001515196.1 tyrosine-type recombinase/integrase -
GTK51_RS23320 (GTK51_23040) 23376..23669 - 294 WP_063116710.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul2 / tet(A) / aph(3'')-Ib / aph(6)-Id / aac(3)-IId / blaTEM-1B / aph(3')-Ia / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / tet(X4) - 1..97200 97200
- inside IScluster/Tn sul2 / tet(A) - 7184..23342 16158


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T146295 WP_001384452.1 NZ_CP047473:c18803-18525 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T146295 NZ_CP062736:4822640-4822981 [Escherichia coli O157:H7]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10321.69 Da        Isoelectric Point: 8.8725

>AT146295 WP_021546940.1 NZ_CP047473:c19072-18803 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMRRKVADK

Download         Length: 270 bp

>AT146295 NZ_CP062736:c4822645-4822569 [Escherichia coli O157:H7]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A1M1SX19

References