Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 56384..56906 Replicon plasmid pDY612-2
Accession NZ_CP047471
Organism Escherichia coli strain DY612

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag GTK51_RS22965 Protein ID WP_000220561.1
Coordinates 56625..56906 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag GTK51_RS22960 Protein ID WP_000121743.1
Coordinates 56384..56635 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTK51_RS22935 (51623) 51623..52483 + 861 WP_063122221.1 hypothetical protein -
GTK51_RS22940 (52504) 52504..52860 - 357 WP_001714234.1 DNA distortion polypeptide 3 -
GTK51_RS22945 (52997) 52997..53443 - 447 WP_001074384.1 hypothetical protein -
GTK51_RS22950 (53447) 53447..54289 - 843 WP_000435064.1 replication initiation protein -
GTK51_RS22955 (54310) 54310..55149 - 840 WP_001352874.1 replication initiation protein -
GTK51_RS22960 (56384) 56384..56635 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
GTK51_RS22965 (56625) 56625..56906 + 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GTK51_RS22970 (57256) 57256..57771 + 516 WP_001025387.1 J domain-containing protein -
GTK51_RS22975 (57845) 57845..58393 + 549 WP_001178641.1 hypothetical protein -
GTK51_RS22980 (58402) 58402..58671 + 270 WP_001006718.1 hypothetical protein -
GTK51_RS22985 (58661) 58661..58903 + 243 WP_000008708.1 hypothetical protein -
GTK51_RS22990 (59036) 59036..59740 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
GTK51_RS22995 (59780) 59780..60307 + 528 WP_260567646.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / cfr / floR - 1..75321 75321
- flank IS/Tn - - 59036..59740 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T146294 WP_000220561.1 NZ_CP047471:56625-56906 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T146294 NZ_CP062736:c4706787-4706473 [Escherichia coli O157:H7]
ATGCAATTTACGGTATACCGCAGTCGCAGTAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT146294 WP_000121743.1 NZ_CP047471:56384-56635 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT146294 NZ_CP062736:c4707023-4706790 [Escherichia coli O157:H7]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References