Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4136716..4137213 Replicon chromosome
Accession NZ_CP047469
Organism Escherichia coli strain DY612

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag GTK51_RS20215 Protein ID WP_001173343.1
Coordinates 4136716..4136889 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag GTK51_RS20220 Protein ID WP_000398619.1
Coordinates 4136917..4137213 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTK51_RS20205 (4133386) 4133386..4134843 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
GTK51_RS20210 (4135080) 4135080..4136597 + 1518 WP_001295074.1 lysine--tRNA ligase -
GTK51_RS20215 (4136716) 4136716..4136889 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
GTK51_RS20220 (4136917) 4136917..4137213 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
GTK51_RS20225 (4137440) 4137440..4137712 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
GTK51_RS20230 (4137724) 4137724..4137954 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
GTK51_RS20235 (4138135) 4138135..4139766 + 1632 WP_001216477.1 sensor histidine kinase -
GTK51_RS20240 (4139763) 4139763..4140482 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T146293 WP_001173343.1 NZ_CP047469:c4136889-4136716 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T146293 NZ_CP062736:4475073-4475333 [Escherichia coli O157:H7]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT146293 WP_000398619.1 NZ_CP047469:c4137213-4136917 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT146293 NZ_CP062736:4474794-4475054 [Escherichia coli O157:H7]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References