Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3942335..3942911 Replicon chromosome
Accession NZ_CP047469
Organism Escherichia coli strain DY612

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag GTK51_RS19230 Protein ID WP_001297643.1
Coordinates 3942624..3942911 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag GTK51_RS19225 Protein ID WP_000063156.1
Coordinates 3942335..3942637 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTK51_RS19210 (3938931) 3938931..3941081 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
GTK51_RS19215 (3941131) 3941131..3941334 + 204 WP_000467859.1 YbdD/YjiX family protein -
GTK51_RS19220 (3941345) 3941345..3942301 + 957 WP_001297640.1 GTPase -
GTK51_RS19225 (3942335) 3942335..3942637 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
GTK51_RS19230 (3942624) 3942624..3942911 - 288 WP_001297643.1 BrnT family toxin Toxin
GTK51_RS19235 (3943110) 3943110..3943807 - 698 Protein_3755 IS1 family transposase -
GTK51_RS19240 (3943864) 3943864..3943959 + 96 Protein_3756 dienelactone hydrolase family protein -
GTK51_RS19245 (3944105) 3944105..3945337 + 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
GTK51_RS19250 (3945378) 3945378..3946658 + 1281 WP_001037400.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - cheD 3933574..3943613 10039


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T146292 WP_001297643.1 NZ_CP047469:c3942911-3942624 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T146292 NZ_CP062736:c4469074-4468676 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT146292 WP_000063156.1 NZ_CP047469:c3942637-3942335 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT146292 NZ_CP062736:c4469370-4469077 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References