Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3447830..3448448 Replicon chromosome
Accession NZ_CP047469
Organism Escherichia coli strain DY612

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GTK51_RS16840 Protein ID WP_001291435.1
Coordinates 3448230..3448448 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GTK51_RS16835 Protein ID WP_000344800.1
Coordinates 3447830..3448204 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTK51_RS16825 (3442919) 3442919..3444112 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GTK51_RS16830 (3444135) 3444135..3447284 + 3150 WP_113402280.1 efflux RND transporter permease AcrB -
GTK51_RS16835 (3447830) 3447830..3448204 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GTK51_RS16840 (3448230) 3448230..3448448 + 219 WP_001291435.1 HHA domain-containing protein Toxin
GTK51_RS16845 (3448620) 3448620..3449171 + 552 WP_000102564.1 maltose O-acetyltransferase -
GTK51_RS16850 (3449287) 3449287..3449757 + 471 WP_000136192.1 YlaC family protein -
GTK51_RS16855 (3449921) 3449921..3451471 + 1551 WP_063501968.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GTK51_RS16860 (3451513) 3451513..3451866 - 354 WP_000878140.1 DUF1428 domain-containing protein -
GTK51_RS16870 (3452245) 3452245..3452556 + 312 WP_000409911.1 MGMT family protein -
GTK51_RS16875 (3452587) 3452587..3453159 - 573 WP_278181044.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T146290 WP_001291435.1 NZ_CP047469:3448230-3448448 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T146290 NZ_CP062736:3755133-3755519 [Escherichia coli O157:H7]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAAGCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT146290 WP_000344800.1 NZ_CP047469:3447830-3448204 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT146290 NZ_CP062736:3755509-3755838 [Escherichia coli O157:H7]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References