Detailed information of TA system TA146289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2477541..2478179
• Replicon: chromosome
• Accession: NZ_CP047469
• Organism: Escherichia coli strain DY612

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: GTK51_RS12135
• Protein ID: WP_000813794.1
• Coordinates: 2478003..2478179 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GTK51_RS12130
• Protein ID: WP_001270286.1
• Coordinates: 2477541..2477957 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTK51_RS12110 (2472693)	2472693..2473634	-	942	WP_001251313.1	ABC transporter permease	-
GTK51_RS12115 (2473635)	2473635..2474648	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
GTK51_RS12120 (2474666)	2474666..2475811	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
GTK51_RS12125 (2476056)	2476056..2477462	-	1407	WP_000760585.1	PLP-dependent aminotransferase family protein	-
GTK51_RS12130 (2477541)	2477541..2477957	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
GTK51_RS12135 (2478003)	2478003..2478179	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
GTK51_RS12140 (2478401)	2478401..2478631	+	231	WP_000494244.1	YncJ family protein	-
GTK51_RS12145 (2478723)	2478723..2480684	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
GTK51_RS12150 (2480757)	2480757..2481293	-	537	WP_000429133.1	DNA-binding transcriptional regulator SutR	-
GTK51_RS12155 (2481346)	2481346..2482560	+	1215	WP_113402181.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2482600..2483865	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T146289 WP_000813794.1 NZ_CP047469:c2478179-2478003 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T146289 NZ_CP062736:3579819-3580106 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT146289 WP_001270286.1 NZ_CP047469:c2477957-2477541 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT146289 NZ_CP062736:3579628-3579819 [Escherichia coli O157:H7]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA

Structures

Toxin

• PDB: 6HPB