Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1271603..1272228 Replicon chromosome
Accession NZ_CP047469
Organism Escherichia coli strain DY612

Toxin (Protein)


Gene name vapC Uniprot ID A0A2J1DF47
Locus tag GTK51_RS06180 Protein ID WP_000911331.1
Coordinates 1271830..1272228 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag GTK51_RS06175 Protein ID WP_000450524.1
Coordinates 1271603..1271830 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTK51_RS06150 (1267406) 1267406..1267876 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
GTK51_RS06155 (1267876) 1267876..1268448 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
GTK51_RS06160 (1268594) 1268594..1269472 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
GTK51_RS06165 (1269489) 1269489..1270523 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
GTK51_RS06170 (1270736) 1270736..1271449 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
GTK51_RS06175 (1271603) 1271603..1271830 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
GTK51_RS06180 (1271830) 1271830..1272228 + 399 WP_000911331.1 type II toxin-antitoxin system VapC family toxin Toxin
GTK51_RS06185 (1272375) 1272375..1273238 + 864 WP_001267507.1 neutral zinc metallopeptidase -
GTK51_RS06190 (1273253) 1273253..1275268 + 2016 WP_001543081.1 tRNA cytosine(34) acetyltransferase TmcA -
GTK51_RS06195 (1275342) 1275342..1276040 + 699 WP_000679812.1 esterase -
GTK51_RS06200 (1276150) 1276150..1276350 - 201 WP_089631931.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14862.19 Da        Isoelectric Point: 9.2216

>T146278 WP_000911331.1 NZ_CP047469:1271830-1272228 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLVELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T146278 NZ_CP062736:751358-751672 [Escherichia coli O157:H7]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTTTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT146278 WP_000450524.1 NZ_CP047469:1271603-1271830 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT146278 NZ_CP062736:751669-752085 [Escherichia coli O157:H7]
ATGATTGCGATTGCCGACATCTTGCATGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACATCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J1DF47

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References