Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 29321..29843 Replicon plasmid pZF34-tetX-114kb
Accession NZ_CP047466
Organism Escherichia coli strain ZF34

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag GTK64_RS22690 Protein ID WP_000220560.1
Coordinates 29321..29602 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag GTK64_RS22695 Protein ID WP_000121743.1
Coordinates 29592..29843 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTK64_RS22660 26043..26702 + 660 WP_164538740.1 IS6 family transposase -
GTK64_RS22665 26801..27109 + 309 Protein_26 IS6-like element IS26 family transposase -
GTK64_RS22670 27174..27638 - 465 Protein_27 restriction endonuclease -
GTK64_RS22675 27847..28158 - 312 WP_001452736.1 hypothetical protein -
GTK64_RS22680 28194..28508 - 315 WP_000734776.1 IncN plasmid KikA protein -
GTK64_RS22685 28549..29246 - 698 Protein_30 IS1 family transposase -
GTK64_RS22690 29321..29602 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GTK64_RS22695 29592..29843 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
GTK64_RS22700 31077..31913 + 837 WP_001050931.1 replication initiation protein -
GTK64_RS22705 31953..32399 + 447 Protein_34 hypothetical protein -
GTK64_RS22710 32467..32892 + 426 WP_000202942.1 hypothetical protein -
GTK64_RS22715 32889..33866 - 978 WP_024245156.1 hypothetical protein -
GTK64_RS22720 33891..34172 - 282 WP_001185482.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(M) / qnrS1 / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / tet(X4) / erm(42) / floR / sul2 - 1..114813 114813


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T146272 WP_000220560.1 NZ_CP047466:c29602-29321 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T146272 NZ_CP062736:283379-283486 [Escherichia coli O157:H7]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT146272 WP_000121743.1 NZ_CP047466:c29843-29592 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT146272 NZ_CP062736:c283331-283265 [Escherichia coli O157:H7]
GGTCTAGGTTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References