Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4167558..4168176 Replicon chromosome
Accession NZ_CP047461
Organism Escherichia coli strain ZF34

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GTK64_RS20025 Protein ID WP_001291435.1
Coordinates 4167558..4167776 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GTK64_RS20030 Protein ID WP_000344800.1
Coordinates 4167802..4168176 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTK64_RS19990 4162847..4163419 + 573 WP_000779831.1 YbaY family lipoprotein -
GTK64_RS19995 4163450..4163761 - 312 WP_000409911.1 MGMT family protein -
GTK64_RS20005 4164140..4164493 + 354 WP_000878140.1 DUF1428 domain-containing protein -
GTK64_RS20010 4164535..4166085 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GTK64_RS20015 4166249..4166719 - 471 WP_000136192.1 YlaC family protein -
GTK64_RS20020 4166835..4167386 - 552 WP_000102564.1 maltose O-acetyltransferase -
GTK64_RS20025 4167558..4167776 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GTK64_RS20030 4167802..4168176 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GTK64_RS20035 4168722..4171871 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
GTK64_RS20040 4171894..4173087 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T146271 WP_001291435.1 NZ_CP047461:c4167776-4167558 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T146271 NZ_CP062735:c2388-1348 [Escherichia coli O157:H7]
ATGGCACAGAACCCGTTTAAAGCACTAAATATCAATATTGACAAGATTGAGTCTGCTCTGACGCAGAATGGCGTCACAAA
CTATTCCTCTAATGTAAAAAACGAAAGAGAAACTCACATATCTGGCACATATAAAGGAATAGACTTCTTAATAAAACTAA
TGCCATCAGGCGGAAATACCACTATCGGAAGAGCGTCTGGGCAAAATAACACTTACTTTGATGAAATCGCCTTGATTATA
AAAGAAAACTGTTTGTATTCAGACACAAAGAACTTTGAATACACCATTCCAAAATTCAGTGATGATGACAGGGCAAATCT
ATTTGAATTTCTTTCTGAAGAGGGGATAACAATAACAGAAGATAATAATAACGATCCTAATTGTAAACACCAATATATTA
TGACCACCAGCAATGGTGACAGGGTCAGGGCAAAAATTTACAAGCGCGGCTCTATTCAATTCCAAGGAAAATACCTTCAA
ATCGCGAGTTTGATTAACGATTTCATGTGCTCAATACTAAACATGAAAGAGATTGTCGAACAAAAAAATAAAGAATTTAA
TGTTGACATAAAAAAAGAAACTATTGAGTCCGAGTTGCATAGCAAACTACCAAAAAGCATCGATAAAATCCATGAAGATA
TCAAAAAACAGCTATCATGCTCGCTAATAATGAAAAAAATAGATGTCGAAATGGAAGATTACTCAACATACTGCTTCTCT
GCATTAAGAGCCATAGAAGGCTTTATATATCAAATACTTAATGATGTTTGCAATCCATCATCATCAAAGAACCTTGGCGA
ATACTTCACTGAAAACAAACCCAAATATATAATCAGAGAAATACACCAAGAAACTATAAATGGTGAAATAGCGGAAGTTT
TGTGTGAATGCTACACTTACTGGCATGAGAACAGGCATGGTTTGTTTCATATGAAACCAGGAATAGCTGACACGAAGACA
ATTAACAAATTAGAATCAATCGCAATCATCGATACCGTTTGCCAATTAATAGATGGTGGCGTAGCTAGGTTGAAATTATG
A

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT146271 WP_000344800.1 NZ_CP047461:c4168176-4167802 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT146271 NZ_CP062735:c1351-971 [Escherichia coli O157:H7]
ATGAAAAAAGATAAAAAATATCAAATAGAAGCAATAAAAAATAAAGATAAAACTTTATTTATTGTCTATGCTACTGATAT
TTATAGCCCGAGCGAATTTTTCTCAAAAATCGAATCCGACTTGAAGAAAAAGAAAAGCAAGGGTGATGTTTTTTTTGATT
TAATAATTCCTAACGGTGGAAAAAAAGATCGTTACGTCTATACGTCATTTAATGGCGAGAAGTTTTCAAGTTACACATTA
AACAAAGTTACGAAAACTGATGAATATAATGATTTATCTGAGCTCTCGGCTTCGTTCTTTAAAAAAAACTTTGATAAGAT
CAACGTAAACCTTCTATCCAAAGCCACATCATTTGCTTTGAAAAAAGGCATTCCAATATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References