Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3616639..3617215 Replicon chromosome
Accession NZ_CP047461
Organism Escherichia coli strain ZF34

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag GTK64_RS17355 Protein ID WP_001297643.1
Coordinates 3616639..3616926 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag GTK64_RS17360 Protein ID WP_000063156.1
Coordinates 3616913..3617215 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTK64_RS17335 3612892..3614172 - 1281 WP_001037400.1 DUF445 domain-containing protein -
GTK64_RS17340 3614213..3615445 - 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
GTK64_RS17345 3615591..3615686 - 96 Protein_3366 dienelactone hydrolase family protein -
GTK64_RS17350 3615743..3616440 + 698 Protein_3367 IS1 family transposase -
GTK64_RS17355 3616639..3616926 + 288 WP_001297643.1 BrnT family toxin Toxin
GTK64_RS17360 3616913..3617215 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
GTK64_RS17365 3617249..3618205 - 957 WP_001297640.1 GTPase -
GTK64_RS17370 3618216..3618419 - 204 WP_000467859.1 YbdD/YjiX family protein -
GTK64_RS17375 3618469..3620619 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T146268 WP_001297643.1 NZ_CP047461:3616639-3616926 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T146268 NZ_CP062734:2065-2340 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT146268 WP_000063156.1 NZ_CP047461:3616913-3617215 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT146268 NZ_CP062734:1781-2065 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References