Detailed information of TA system TA146267

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 3449870..3450468
• Replicon: chromosome
• Accession: NZ_CP047461
• Organism: Escherichia coli strain ZF34

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: GTK64_RS16520
• Protein ID: WP_053270478.1
• Coordinates: 3450091..3450468 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: GTK64_RS16515
• Protein ID: WP_001603498.1
• Coordinates: 3449870..3450091 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTK64_RS16485	3445555..3445707	-	153	WP_001696589.1	DUF905 family protein	-
GTK64_RS16490	3445713..3446390	-	678	WP_063091580.1	hypothetical protein	-
GTK64_RS16495	3446509..3447393	-	885	WP_023563932.1	50S ribosome-binding GTPase	-
GTK64_RS16500	3447499..3448449	+	951	WP_000157733.1	hypothetical protein	-
GTK64_RS16505	3448562..3449259	-	698	Protein_3203	IS1-like element IS1A family transposase	-
GTK64_RS16510	3449317..3449685	+	369	Protein_3204	type 1 fimbrial protein	-
GTK64_RS16515	3449870..3450091	+	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GTK64_RS16520	3450091..3450468	+	378	WP_053270478.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
GTK64_RS16525	3450578..3450793	-	216	Protein_3207	transposase	-
GTK64_RS16530	3450979..3452241	-	1263	WP_063078140.1	integrase arm-type DNA-binding domain-containing protein	-
GTK64_RS16540	3452621..3453196	-	576	WP_001188520.1	transcriptional regulator	-
GTK64_RS16545	3453233..3454930	-	1698	WP_164538536.1	protein-disulfide reductase DsbD	-
GTK64_RS16550	3454906..3455244	-	339	WP_000883400.1	divalent cation tolerance protein CutA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3447499..3453196	5697
	-	inside	IScluster/Tn	-	-	3448562..3450793	2231

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13419.20 Da
• Isoelectric Point: 6.7260

>T146267 WP_053270478.1 NZ_CP047461:3450091-3450468 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMSDSGRAEAIIGRVQARVAYEEITGLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T146267 NZ_CP062733:c5053971-5053798 [Escherichia coli O157:H7]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT146267 WP_001603498.1 NZ_CP047461:3449870-3450091 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT146267 NZ_CP062733:c5054295-5053999 [Escherichia coli O157:H7]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: L4J1C0