Detailed information of TA system TA146266

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3442360..3443195
• Replicon: chromosome
• Accession: NZ_CP047461
• Organism: Escherichia coli strain ZF34

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: GTK64_RS16455
• Protein ID: WP_000854821.1
• Coordinates: 3442360..3442737 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: GTK64_RS16460
• Protein ID: WP_001285610.1
• Coordinates: 3442827..3443195 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTK64_RS16435	3438727..3440265	-	1539	WP_001187177.1	lysine decarboxylation/transport transcriptional activator CadC	-
GTK64_RS16440	3441014..3441856	-	843	WP_001280501.1	DUF4942 domain-containing protein	-
GTK64_RS16445	3441941..3442138	-	198	WP_023563519.1	DUF957 domain-containing protein	-
GTK64_RS16450	3442166..3442363	-	198	Protein_3192	hypothetical protein	-
GTK64_RS16455	3442360..3442737	-	378	WP_000854821.1	type IV toxin-antitoxin system toxin CbtA	Toxin
GTK64_RS16460	3442827..3443195	-	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GTK64_RS16465	3443275..3443496	-	222	WP_000692350.1	DUF987 domain-containing protein	-
GTK64_RS16470	3443583..3444059	-	477	WP_063091579.1	RadC family protein	-
GTK64_RS16475	3444075..3444554	-	480	WP_000860074.1	antirestriction protein	-
GTK64_RS16480	3444636..3445454	-	819	WP_001234688.1	DUF945 domain-containing protein	-
GTK64_RS16485	3445555..3445707	-	153	WP_001696589.1	DUF905 family protein	-
GTK64_RS16490	3445713..3446390	-	678	WP_063091580.1	hypothetical protein	-
GTK64_RS16495	3446509..3447393	-	885	WP_023563932.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T146266 WP_000854821.1 NZ_CP047461:c3442737-3442360 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T146266 NZ_CP062733:c4957158-4956808 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT146266 WP_001285610.1 NZ_CP047461:c3443195-3442827 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT146266 NZ_CP062733:c4957403-4957152 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54