Detailed information of TA system TA146260

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1926964..1927547
• Replicon: chromosome
• Accession: NZ_CP047461
• Organism: Escherichia coli strain ZF34

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: S1EZP4
• Locus tag: GTK64_RS09350
• Protein ID: WP_000254738.1
• Coordinates: 1926964..1927299 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S1P3W5
• Locus tag: GTK64_RS09355
• Protein ID: WP_000581937.1
• Coordinates: 1927299..1927547 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTK64_RS09335	1922851..1924149	-	1299	WP_000036723.1	phosphopyruvate hydratase	-
GTK64_RS09340	1924237..1925874	-	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
GTK64_RS09345	1926102..1926893	-	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
GTK64_RS09350	1926964..1927299	-	336	WP_000254738.1	endoribonuclease MazF	Toxin
GTK64_RS09355	1927299..1927547	-	249	WP_000581937.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
GTK64_RS09360	1927625..1929859	-	2235	WP_000226815.1	GTP pyrophosphokinase	-
GTK64_RS09365	1929907..1931208	-	1302	WP_001556187.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12098.04 Da
• Isoelectric Point: 8.2618

>T146260 WP_000254738.1 NZ_CP047461:c1927299-1926964 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T146260 NZ_CP062733:3755104-3755490 [Escherichia coli O157:H7]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAAGCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9355.74 Da
• Isoelectric Point: 4.4665

>AT146260 WP_000581937.1 NZ_CP047461:c1927547-1927299 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT146260 NZ_CP062733:3755480-3755809 [Escherichia coli O157:H7]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CK9
• PDB: 5CKH
• PDB: 5CO7
• PDB: 3NFC
• PDB: 5CR2
• PDB: 5CKB
• PDB: 5CQY
• PDB: 5CKD
• PDB: 5CKE
• PDB: 5CKF

Antitoxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CQY
• PDB: 1MVF
• PDB: 2MRN
• PDB: 2MRU
• AlphaFold DB: A0A7U9LMB4