Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 87764..88286 Replicon plasmid pZF31-tetX-119kb
Accession NZ_CP047460
Organism Escherichia coli strain ZF31

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag GTK48_RS22980 Protein ID WP_000220560.1
Coordinates 87764..88045 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag GTK48_RS22985 Protein ID WP_000121743.1
Coordinates 88035..88286 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTK48_RS22950 84487..85191 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
GTK48_RS22955 85244..85552 + 309 Protein_81 IS6-like element IS26 family transposase -
GTK48_RS22960 85617..86081 - 465 Protein_82 restriction endonuclease -
GTK48_RS22965 86290..86601 - 312 WP_001452736.1 hypothetical protein -
GTK48_RS22970 86637..86951 - 315 WP_000734776.1 IncN plasmid KikA protein -
GTK48_RS22975 86992..87689 - 698 Protein_85 IS1 family transposase -
GTK48_RS22980 87764..88045 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GTK48_RS22985 88035..88286 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
GTK48_RS22990 89520..90356 + 837 WP_001050931.1 replication initiation protein -
GTK48_RS22995 90396..90842 + 447 Protein_89 hypothetical protein -
GTK48_RS23000 90910..91335 + 426 WP_000202942.1 hypothetical protein -
GTK48_RS23005 91332..92309 - 978 WP_024245156.1 hypothetical protein -
GTK48_RS23010 92334..92615 - 282 WP_001185482.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(X4) / erm(42) / floR / sul2 / tet(M) / tet(A) / qnrS1 / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 - 1..118248 118248


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T146249 WP_000220560.1 NZ_CP047460:c88045-87764 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T146249 NZ_CP062733:946467-946874 [Escherichia coli O157:H7]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT146249 WP_000121743.1 NZ_CP047460:c88286-88035 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT146249 NZ_CP062733:946220-946486 [Escherichia coli O157:H7]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References