Detailed information of TA system TA146244

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 3449193..3449791
• Replicon: chromosome
• Accession: NZ_CP047455
• Organism: Escherichia coli strain ZF31

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: GTK48_RS16505
• Protein ID: WP_053270478.1
• Coordinates: 3449414..3449791 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: GTK48_RS16500
• Protein ID: WP_001603498.1
• Coordinates: 3449193..3449414 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTK48_RS16470	3444878..3445030	-	153	WP_001696589.1	DUF905 family protein	-
GTK48_RS16475	3445036..3445713	-	678	WP_063091580.1	hypothetical protein	-
GTK48_RS16480	3445832..3446716	-	885	WP_023563932.1	50S ribosome-binding GTPase	-
GTK48_RS16485	3446822..3447772	+	951	WP_000157733.1	hypothetical protein	-
GTK48_RS16490	3447885..3448582	-	698	Protein_3203	IS1-like element IS1A family transposase	-
GTK48_RS16495	3448640..3449008	+	369	Protein_3204	type 1 fimbrial protein	-
GTK48_RS16500	3449193..3449414	+	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GTK48_RS16505	3449414..3449791	+	378	WP_053270478.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
GTK48_RS16510	3449901..3450116	-	216	Protein_3207	transposase	-
GTK48_RS16515	3450302..3451564	-	1263	WP_063078140.1	integrase arm-type DNA-binding domain-containing protein	-
GTK48_RS16525	3451944..3452519	-	576	WP_001188520.1	transcriptional regulator	-
GTK48_RS16530	3452556..3454253	-	1698	WP_164538536.1	protein-disulfide reductase DsbD	-
GTK48_RS16535	3454229..3454567	-	339	WP_000883400.1	divalent cation tolerance protein CutA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3446822..3452519	5697
	-	inside	IScluster/Tn	-	-	3447885..3450116	2231

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13419.20 Da
• Isoelectric Point: 6.7260

>T146244 WP_053270478.1 NZ_CP047455:3449414-3449791 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMSDSGRAEAIIGRVQARVAYEEITGLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T146244 NZ_CP062733:408067-408594 [Escherichia coli O157:H7]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT146244 WP_001603498.1 NZ_CP047455:3449193-3449414 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT146244 NZ_CP062733:407794-408060 [Escherichia coli O157:H7]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Antitoxin

• AlphaFold DB: L4J1C0